The ability of the zebrafish heart to regenerate following injury makes it a valuable model to deduce why this capability in mammals is limited to early neonatal stages. Although metabolic reprogramming and glycosylation remodeling have emerged as key aspects in many biological processes, how they may trigger a cardiac regenerative response in zebrafish is still a crucial question. Here, by using an up-to-date panel of transcriptomic, proteomic and glycomic approaches, we identify a metabolic switch from mitochondrial oxidative phosphorylation to glycolysis associated with membrane glycosylation remodeling during heart regeneration. Importantly, we establish the N- and O-linked glycan structural repertoire of the regenerating zebrafish heart, and link alterations in both sialylation and high mannose structures across the phases of regeneration. Our results show that metabolic reprogramming and glycan structural remodeling are potential drivers of tissue regeneration after cardiac injury, providing the biological rationale to develop novel therapeutics to elicit heart regeneration in mammals.

Spelat, R., Ferro, F., Contessotto, P., Aljaabary, A., Martin-Saldana, S., Jin, C., et al. (2022). Metabolic reprogramming and membrane glycan remodeling as potential drivers of zebrafish heart regeneration. COMMUNICATIONS BIOLOGY, 5(1) [10.1038/s42003-022-04328-2].

Metabolic reprogramming and membrane glycan remodeling as potential drivers of zebrafish heart regeneration

Magni F.;Chinello C.;
2022

Abstract

The ability of the zebrafish heart to regenerate following injury makes it a valuable model to deduce why this capability in mammals is limited to early neonatal stages. Although metabolic reprogramming and glycosylation remodeling have emerged as key aspects in many biological processes, how they may trigger a cardiac regenerative response in zebrafish is still a crucial question. Here, by using an up-to-date panel of transcriptomic, proteomic and glycomic approaches, we identify a metabolic switch from mitochondrial oxidative phosphorylation to glycolysis associated with membrane glycosylation remodeling during heart regeneration. Importantly, we establish the N- and O-linked glycan structural repertoire of the regenerating zebrafish heart, and link alterations in both sialylation and high mannose structures across the phases of regeneration. Our results show that metabolic reprogramming and glycan structural remodeling are potential drivers of tissue regeneration after cardiac injury, providing the biological rationale to develop novel therapeutics to elicit heart regeneration in mammals.
Articolo in rivista - Articolo scientifico
cardiac injury; tissue regeneration
English
13-dic-2022
2022
5
1
1365
open
Spelat, R., Ferro, F., Contessotto, P., Aljaabary, A., Martin-Saldana, S., Jin, C., et al. (2022). Metabolic reprogramming and membrane glycan remodeling as potential drivers of zebrafish heart regeneration. COMMUNICATIONS BIOLOGY, 5(1) [10.1038/s42003-022-04328-2].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/454258
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