Scope: Bifidobacteria play a role in intestinal homeostasis but molecular mechanisms remain underinvestigated. The aim of this study was to assess if probiotic Bifidobacterium strains alter expression of intestinal microRNA and downstream target gene response. Methods and results: The expression of miR-148a and its validated target endothelial PAS domain protein 1 (EPAS1) was analyzed in Caco-2 cells and mice cecum in response to Bifidobacterium bifidum MIMBb75, B. bifidum NCC390, or Bifidobacterium longum NCC2705. In vitro, exposure to B. bifidum MIMBb75, but not to B. bifidum NCC390 or B. longum NCC2705, increased the expression of miR-148a after 1 and 4 h (p < 0.01), but not after 24 h. In vivo, B. bifidum MIMBb75 administration to C57BL/6J mice increased miR-148a expression in the cecum after 2 but not 14 days (p < 0.05). The increase in miR-148a was accompanied by a decrease in EPAS1 expression in Caco-2 cells and cecum (p < 0.05). Silencing of miR-148a reversed B. bifidum MIMBb75 dependent downregulation of EPAS1. Conclusion: This study shows an early response of intestinal cells to B. bifidum MIMBb75 through miR-148a modulation. This brings a new concept of strain- and time-dependent bifidobacteria–host crosstalk via microRNA. Probiotic B. bifidum MIMBb75 may help attenuating EPAS1 overexpression associated with intestinal inflammation.

Taibi, A., Singh, N., Chen, J., Arioli, S., Guglielmetti, S., Comelli, E. (2017). Time- and strain-specific downregulation of intestinal EPAS1 via miR-148a by Bifidobacterium bifidum. MOLECULAR NUTRITION & FOOD RESEARCH, 61(5 (May 2017)), 1-8 [10.1002/mnfr.201600596].

Time- and strain-specific downregulation of intestinal EPAS1 via miR-148a by Bifidobacterium bifidum

Guglielmetti S.;
2017

Abstract

Scope: Bifidobacteria play a role in intestinal homeostasis but molecular mechanisms remain underinvestigated. The aim of this study was to assess if probiotic Bifidobacterium strains alter expression of intestinal microRNA and downstream target gene response. Methods and results: The expression of miR-148a and its validated target endothelial PAS domain protein 1 (EPAS1) was analyzed in Caco-2 cells and mice cecum in response to Bifidobacterium bifidum MIMBb75, B. bifidum NCC390, or Bifidobacterium longum NCC2705. In vitro, exposure to B. bifidum MIMBb75, but not to B. bifidum NCC390 or B. longum NCC2705, increased the expression of miR-148a after 1 and 4 h (p < 0.01), but not after 24 h. In vivo, B. bifidum MIMBb75 administration to C57BL/6J mice increased miR-148a expression in the cecum after 2 but not 14 days (p < 0.05). The increase in miR-148a was accompanied by a decrease in EPAS1 expression in Caco-2 cells and cecum (p < 0.05). Silencing of miR-148a reversed B. bifidum MIMBb75 dependent downregulation of EPAS1. Conclusion: This study shows an early response of intestinal cells to B. bifidum MIMBb75 through miR-148a modulation. This brings a new concept of strain- and time-dependent bifidobacteria–host crosstalk via microRNA. Probiotic B. bifidum MIMBb75 may help attenuating EPAS1 overexpression associated with intestinal inflammation.
Articolo in rivista - Articolo scientifico
Bifidobacterium bifidum; EPAS1; Intestine; MicroRNA; Probiotic; Time;
English
24-nov-2016
2017
61
5 (May 2017)
1
8
1600596
partially_open
Taibi, A., Singh, N., Chen, J., Arioli, S., Guglielmetti, S., Comelli, E. (2017). Time- and strain-specific downregulation of intestinal EPAS1 via miR-148a by Bifidobacterium bifidum. MOLECULAR NUTRITION & FOOD RESEARCH, 61(5 (May 2017)), 1-8 [10.1002/mnfr.201600596].
File in questo prodotto:
File Dimensione Formato  
Taibi-2017-Molecul Nutr Food Res-AAM.pdf

accesso aperto

Descrizione: Research Article
Tipologia di allegato: Author’s Accepted Manuscript, AAM (Post-print)
Licenza: Altro
Dimensione 1.08 MB
Formato Adobe PDF
1.08 MB Adobe PDF Visualizza/Apri
Taibi-2017-Molecul Nutr Food Res-VoR.pdf

Solo gestori archivio

Descrizione: Research Article
Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Tutti i diritti riservati
Dimensione 533.55 kB
Formato Adobe PDF
533.55 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/452886
Citazioni
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 13
Social impact