Objectives: The work shows the effect of counting rules, such as analysis magnification and asbestos fiber dimension to be count (with length ≥5 µm or also asbestos fibers with length <5 µm) in the lung asbestos fiber burden analysis for legal medicine evaluations. Methods: On the same lung tissue samples, two different analyses were carried out to count any asbestos fibers with length ≥1 µm and with length ≥5 µm. Results of the amphibole burden of the two analyses were compared by linear regression analysis on log10-transformed values. Results: The analysis should be carried out at an appropriate magnification and on samples prepared in such a way as they allow the counting of very fine fibers. If the analysis is limited to the asbestos fibers with length ≥5 µm, there is a high risk of not detecting possible residual chrysotile fiber burden and thinner crocidolite asbestos fibers. Conclusions: On average we estimated that 1 amphibole fiber with length ≥5 µm corresponds to ∼8 amphibole fibers with length ≥1 µm in the lung. The values of the Helsinki criteria should be updated taking this into account.

Somigliana, A., Barbieri, P., Cavallo, A., Colombo, R., Consonni, D., Mirabelli, D. (2023). Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations. INHALATION TOXICOLOGY, 35(11-12), 300-307 [10.1080/08958378.2023.2285789].

Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations

Cavallo, A;
2023

Abstract

Objectives: The work shows the effect of counting rules, such as analysis magnification and asbestos fiber dimension to be count (with length ≥5 µm or also asbestos fibers with length <5 µm) in the lung asbestos fiber burden analysis for legal medicine evaluations. Methods: On the same lung tissue samples, two different analyses were carried out to count any asbestos fibers with length ≥1 µm and with length ≥5 µm. Results of the amphibole burden of the two analyses were compared by linear regression analysis on log10-transformed values. Results: The analysis should be carried out at an appropriate magnification and on samples prepared in such a way as they allow the counting of very fine fibers. If the analysis is limited to the asbestos fibers with length ≥5 µm, there is a high risk of not detecting possible residual chrysotile fiber burden and thinner crocidolite asbestos fibers. Conclusions: On average we estimated that 1 amphibole fiber with length ≥5 µm corresponds to ∼8 amphibole fibers with length ≥1 µm in the lung. The values of the Helsinki criteria should be updated taking this into account.
Articolo in rivista - Articolo scientifico
Asbestos; fibre length; Helsinki Consensus Report; lung burden analysis; SEM;
English
23-nov-2023
2023
35
11-12
300
307
reserved
Somigliana, A., Barbieri, P., Cavallo, A., Colombo, R., Consonni, D., Mirabelli, D. (2023). Lung asbestos fiber burden analysis: effects of the counting rules for legal medicine evaluations. INHALATION TOXICOLOGY, 35(11-12), 300-307 [10.1080/08958378.2023.2285789].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/451699
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