Recently, we demonstrated that TLQP-21 triggers lipolysis and induces resistance to obesity by reducing fat accumulation [1]. TLQP-21 is a 21 amino acid peptide cleavage product of the neuroprotein VGF and was first identified in rat brain. Although TLQP-21 biological activity and its molecular signaling is under active investigation, a receptor for TLQP-21 has not yet been characterized. We now demonstrate that TLQP-21 stimulates intracellular calcium mobilization in CHO cells. Furthermore, using Atomic Force Microscopy (AFM), we also provide evidence of TLQP-21 binding-site characteristics in CHO cells. AFM was used in force mapping mode equipped with a cantilever suitably functionalized with TLQP-21. Attraction of this functionalized probe to the cell surface was specific and consistent with the biological activity of TLQP-21; by contrast, there was no attraction of a probe functionalized with biologically inactive analogues. We detected interaction of the peptide with the binding-site by scanning the cell surface with the cantilever tip. The attractive force between TLQP-21 and its binding site was measured, statistically analyzed and quantified at approximately 40 pN on average, indicating a single class of binding sites. Furthermore we observed that the distribution of these binding sites on the surface was relatively uniform. © 2012 Elsevier B.V. All rights reserved.

Cassina, V., Torsello, A., Tempestini, A., Salerno, D., Brogioli, D., Tamiazzo, L., et al. (2013). Biophysical characterization of a binding site for TLQP-21, a naturally occurring peptide which induces resistance to obesity. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1828(2), 455-460 [10.1016/j.bbamem.2012.10.023].

Biophysical characterization of a binding site for TLQP-21, a naturally occurring peptide which induces resistance to obesity

CASSINA, VALERIA;TORSELLO, ANTONIO BIAGIO;TEMPESTINI, ALESSIA;SALERNO, DOMENICO;BROGIOLI, DORIANO COSTANTINO;TAMIAZZO, LAURA;BRESCIANI, ELENA;RIZZI, LAURA;LOCATELLI, VITTORIO;MANTEGAZZA, FRANCESCO
2013

Abstract

Recently, we demonstrated that TLQP-21 triggers lipolysis and induces resistance to obesity by reducing fat accumulation [1]. TLQP-21 is a 21 amino acid peptide cleavage product of the neuroprotein VGF and was first identified in rat brain. Although TLQP-21 biological activity and its molecular signaling is under active investigation, a receptor for TLQP-21 has not yet been characterized. We now demonstrate that TLQP-21 stimulates intracellular calcium mobilization in CHO cells. Furthermore, using Atomic Force Microscopy (AFM), we also provide evidence of TLQP-21 binding-site characteristics in CHO cells. AFM was used in force mapping mode equipped with a cantilever suitably functionalized with TLQP-21. Attraction of this functionalized probe to the cell surface was specific and consistent with the biological activity of TLQP-21; by contrast, there was no attraction of a probe functionalized with biologically inactive analogues. We detected interaction of the peptide with the binding-site by scanning the cell surface with the cantilever tip. The attractive force between TLQP-21 and its binding site was measured, statistically analyzed and quantified at approximately 40 pN on average, indicating a single class of binding sites. Furthermore we observed that the distribution of these binding sites on the surface was relatively uniform. © 2012 Elsevier B.V. All rights reserved.
Articolo in rivista - Articolo scientifico
TLQP-21; VGF; Ligand–receptor;Atomic force Microscopy (AFM); CHO; Fluorescence
English
455
460
6
Cassina, V., Torsello, A., Tempestini, A., Salerno, D., Brogioli, D., Tamiazzo, L., et al. (2013). Biophysical characterization of a binding site for TLQP-21, a naturally occurring peptide which induces resistance to obesity. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1828(2), 455-460 [10.1016/j.bbamem.2012.10.023].
Cassina, V; Torsello, A; Tempestini, A; Salerno, D; Brogioli, D; Tamiazzo, L; Bresciani, E; Martinez, J; Fehrentz, J; Verdie, P; Omeljaniuk, R; Possenti, R; Rizzi, L; Locatelli, V; Mantegazza, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/45057
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