In vitro systems make for rapid identification of xenobiotic effects and can be used to study cellular and subcellular toxicity mechanisms. In this report the metabolic competence of two human-derived cell lines, a hepatic (Hep G2) and a pulmonary one (A549) was tested. In the two cell systems the capability to activate Benzo[a]Pyrene through the cytochrome P450 enzyme system and to form reactive metabolites was analysed. H-3-BaP and the scintillation counting analysis were used to show the differences of the metabolic activity in Hep G2 and A549. A similar time course of 3H-BaP uptake was observed in the cell systems. Nevertheless, in the two cell lines the distribution of radioactive metabolites seemed to reflect a specific tissue response to toxicity.
|Citazione:||Urani, C., Doldi, M., Crippa, S., & Camatini, M. (1998). Human-derived cell lines to study xenobiotic metabolism. CHEMOSPHERE, 37, 2785-2795.|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Titolo:||Human-derived cell lines to study xenobiotic metabolism|
|Autori:||Urani, C; Doldi, M; Crippa, S; Camatini, M|
|Data di pubblicazione:||1998|
|Appare nelle tipologie:||01 - Articolo su rivista|