This paper describes the use of two in vitro systems (stage 35 Xenopus laevis embryos and the human hepatoblastoma cell line, Hep G2) to study effects of some environmental contaminants (benzo[a]pyrene, copper and zinc), which are representative of chemicals with different cell targets and mechanisms of action. The ability to activate benzo[a]pyrene and to metabolise it with the cytochrome P4501A isozyme were demonstrated in both in vitro systems by assessing the formation of water-soluble and protein-bound benzo[a]pyrene metabolites and by immunochemical analysis. In X. laevis embryos, the formation of DNA adducts demonstrated the ability to produce benzo[a]pyrene reactive metabolites. Moreover, in Hep G2 cells, the cytoskeletal protein, tubulin, and the reduced form of glutathione proved to be the cellular targets of copper and zinc toxicity. In response to metal-induced stress in Hep G2 cells, there was a cytoplasmic reorganisation of heat shock protein, Hsp 70. In conclusion, the in vitro systems provide for a rapid evaluation of heterogeneous compounds such as benzo[a]pyrene and heavy metals that differ in toxic potency and mechanisms of action. They could also be used to study the mechanisms of toxic action and to identify specific cellular and molecular targets.

Camatini, M., Bonfanti, P., Colombo, A., Urani, C., & Crippa, S. (1999). Cellular and molecular targets of benzo[a]pyrene and metal toxicity in Xenopus laevis embryos and in Hep G2 cells. ATLA. ALTERNATIVES TO LABORATORY ANIMALS, 27, 325-337.

Cellular and molecular targets of benzo[a]pyrene and metal toxicity in Xenopus laevis embryos and in Hep G2 cells

CAMATINI, MARINA CARLA;BONFANTI, PATRIZIA;COLOMBO, ANITA EMILIA;URANI, CHIARA;
1999

Abstract

This paper describes the use of two in vitro systems (stage 35 Xenopus laevis embryos and the human hepatoblastoma cell line, Hep G2) to study effects of some environmental contaminants (benzo[a]pyrene, copper and zinc), which are representative of chemicals with different cell targets and mechanisms of action. The ability to activate benzo[a]pyrene and to metabolise it with the cytochrome P4501A isozyme were demonstrated in both in vitro systems by assessing the formation of water-soluble and protein-bound benzo[a]pyrene metabolites and by immunochemical analysis. In X. laevis embryos, the formation of DNA adducts demonstrated the ability to produce benzo[a]pyrene reactive metabolites. Moreover, in Hep G2 cells, the cytoskeletal protein, tubulin, and the reduced form of glutathione proved to be the cellular targets of copper and zinc toxicity. In response to metal-induced stress in Hep G2 cells, there was a cytoplasmic reorganisation of heat shock protein, Hsp 70. In conclusion, the in vitro systems provide for a rapid evaluation of heterogeneous compounds such as benzo[a]pyrene and heavy metals that differ in toxic potency and mechanisms of action. They could also be used to study the mechanisms of toxic action and to identify specific cellular and molecular targets.
Articolo in rivista - Articolo scientifico
metals; benzo[a]pyrene; Xenopus laevis embryos; Hep G2 cells; cell targets; POLYCYCLIC AROMATIC-HYDROCARBONS; CARCINOGEN-DNA ADDUCTS; HEAT-SHOCK PROTEINS; IN-VITRO; METABOLIC-ACTIVATION; CYTOSKELETAL ALTERATIONS; DEVELOPMENTAL TOXICITY; OXIDATIVE STRESS; DIOL-EPOXIDE; COPPER IONS
English
325
337
Camatini, M., Bonfanti, P., Colombo, A., Urani, C., & Crippa, S. (1999). Cellular and molecular targets of benzo[a]pyrene and metal toxicity in Xenopus laevis embryos and in Hep G2 cells. ATLA. ALTERNATIVES TO LABORATORY ANIMALS, 27, 325-337.
Camatini, M; Bonfanti, P; Colombo, A; Urani, C; Crippa, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/4474
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