Substantial progress has been made over the last several years in the development of protocols for the isolation of large numbers of dendritic cells (DCs) from different tissues and their short-term culture. Indeed, several stable DC lines and clones have been established from various tissues of mice and humans, providing useful experimental tools for studying the biology of DCs at both molecular and biochemical levels and for the establishment of new DC-based immunotherapies. In this chapter, we will describe the development of long-term DC lines that maintain the growth factor dependence and their immature functional state, thus providing a unique opportunity to study the mechanisms of the initiation of the immune response to infectious agents

Mortellaro, A., Urbano, M., Citterio, S., Foti, M., Granucci, F., Castagnoli, P. (2009). Generation of murine growth factor dependent long-term dendritic cell lines to investigate host-parasite interactions. In N.E. Reiner (a cura di), Macrophages and Dendritic Cells Methods and Protocols (pp. 17-27). Humana Press [10.1007/978-1-59745-396-7_2].

Generation of murine growth factor dependent long-term dendritic cell lines to investigate host-parasite interactions

MORTELLARO, ALESSANDRA ROSA;URBANO, MATTEO;CITTERIO, STEFANIA;FOTI, MARIA;GRANUCCI, FRANCESCA;CASTAGNOLI, PAOLA
2009

Abstract

Substantial progress has been made over the last several years in the development of protocols for the isolation of large numbers of dendritic cells (DCs) from different tissues and their short-term culture. Indeed, several stable DC lines and clones have been established from various tissues of mice and humans, providing useful experimental tools for studying the biology of DCs at both molecular and biochemical levels and for the establishment of new DC-based immunotherapies. In this chapter, we will describe the development of long-term DC lines that maintain the growth factor dependence and their immature functional state, thus providing a unique opportunity to study the mechanisms of the initiation of the immune response to infectious agents
Capitolo o saggio
Mouse growth factor; dendritic cell lines; host-parasite; Time Factors; Cell Line; Granulocyte-Macrophage Colony-Stimulating Factor; Culture Media, Conditioned; Phenotype; Cell Culture Techniques; Animals; Receptors, Cell Surface; Dendritic Cells; Spleen; Bacteria; NIH 3T3 Cells; Bacterial Infections; Cell Differentiation; Mice; Host-Parasite Interactions; Bone Marrow Cells
English
Macrophages and Dendritic Cells Methods and Protocols
Reiner, NE
2009
978-1-58829-972-7
531
Humana Press
17
27
Mortellaro, A., Urbano, M., Citterio, S., Foti, M., Granucci, F., Castagnoli, P. (2009). Generation of murine growth factor dependent long-term dendritic cell lines to investigate host-parasite interactions. In N.E. Reiner (a cura di), Macrophages and Dendritic Cells Methods and Protocols (pp. 17-27). Humana Press [10.1007/978-1-59745-396-7_2].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/44495
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