Intrauterine infection/inflammation (IUI) is a frequent complication of pregnancy leading to preterm labor and fetal infllammation. How inflammation is modulated at the maternal-fetal interface is unresolved. We compared transcriptomics of amnion (a fetal tissue in contact with amniotic fluid) in a preterm rhesus macaque model of IUI-induced by lypopolysaccharide with human cohorts of chorioamnionitis. Bulk RNA-seq amnion transcriptomic profiles were remarkably similar in both rhesus and human subjects and revealed that induction of key labor-mediating genes such as IL1 and IL6 was dependent on NF-kB signaling and reversed by the anti-TNF antibody Adalimumab. Inhibition of collagen biosynthesis by IUI was partially restored by Adalimumab. Interestingly, single-cell transcriptomics, flow cytometry, and immuno-histology demonstrated that a subset of amnion mesenchymal cells (AMCs) increase CD14 and other myeloid cell markers during IUI both in the human and Rhesus macaque. Our data suggest that CD14+ AMCs represent activated AMCs at the maternal-fetal interface.

Presicce, P., Cappelletti, M., Morselli, M., Ma, F., Senthamaraikannan, P., Protti, G., et al. (2023). Amnion responses to intrauterine inflammation and effects of inhibition of TNF-signaling in preterm Rhesus macaque. ISCIENCE, 26(11 (17 November 2023)) [10.1016/j.isci.2023.108118].

Amnion responses to intrauterine inflammation and effects of inhibition of TNF-signaling in preterm Rhesus macaque

Protti, Giulia;
2023

Abstract

Intrauterine infection/inflammation (IUI) is a frequent complication of pregnancy leading to preterm labor and fetal infllammation. How inflammation is modulated at the maternal-fetal interface is unresolved. We compared transcriptomics of amnion (a fetal tissue in contact with amniotic fluid) in a preterm rhesus macaque model of IUI-induced by lypopolysaccharide with human cohorts of chorioamnionitis. Bulk RNA-seq amnion transcriptomic profiles were remarkably similar in both rhesus and human subjects and revealed that induction of key labor-mediating genes such as IL1 and IL6 was dependent on NF-kB signaling and reversed by the anti-TNF antibody Adalimumab. Inhibition of collagen biosynthesis by IUI was partially restored by Adalimumab. Interestingly, single-cell transcriptomics, flow cytometry, and immuno-histology demonstrated that a subset of amnion mesenchymal cells (AMCs) increase CD14 and other myeloid cell markers during IUI both in the human and Rhesus macaque. Our data suggest that CD14+ AMCs represent activated AMCs at the maternal-fetal interface.
Articolo in rivista - Articolo scientifico
amnion, intrauterine infection, rhesus macaque
English
6-ott-2023
2023
26
11 (17 November 2023)
108118
none
Presicce, P., Cappelletti, M., Morselli, M., Ma, F., Senthamaraikannan, P., Protti, G., et al. (2023). Amnion responses to intrauterine inflammation and effects of inhibition of TNF-signaling in preterm Rhesus macaque. ISCIENCE, 26(11 (17 November 2023)) [10.1016/j.isci.2023.108118].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/443919
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