The development of human tissue models for regenerative medicine and animal-free drug screening requires glycosylated biomaterials such as collagen. An easy and fast biomaterial glycosylation method exploiting Horseradish Peroxidase (HRP) phenol coupling reaction is proposed. The protocol is adaptable to any polymer functionalized with phenol residues or tyrosine containing proteins. As a model the tyrosine residues on collagen films were functionalized with salidroside, a natural β-glucoside with a phenol in the aglycone. Scanning Electron Microscope (SEM) and contact angle analysis revealed the influence of glycosylation on the sample's morphology and wettability. Preliminary biological evaluation showed the cytocompatibility of the glucosylated collagen films.

Cadamuro, F., Ferrario, M., Akbari, R., Antonini, C., Nicotra, F., Russo, L. (2023). Tyrosine glucosylation of collagen films exploiting Horseradish Peroxidase (HRP). CARBOHYDRATE RESEARCH, 533(November 2023) [10.1016/j.carres.2023.108938].

Tyrosine glucosylation of collagen films exploiting Horseradish Peroxidase (HRP)

Cadamuro, F;Ferrario, M;Akbari, R;Antonini, C;Nicotra, F;Russo, L
2023

Abstract

The development of human tissue models for regenerative medicine and animal-free drug screening requires glycosylated biomaterials such as collagen. An easy and fast biomaterial glycosylation method exploiting Horseradish Peroxidase (HRP) phenol coupling reaction is proposed. The protocol is adaptable to any polymer functionalized with phenol residues or tyrosine containing proteins. As a model the tyrosine residues on collagen films were functionalized with salidroside, a natural β-glucoside with a phenol in the aglycone. Scanning Electron Microscope (SEM) and contact angle analysis revealed the influence of glycosylation on the sample's morphology and wettability. Preliminary biological evaluation showed the cytocompatibility of the glucosylated collagen films.
Articolo in rivista - Articolo scientifico
Biomaterials glycosylation; Collagen; Glycosignature; HRP;
English
9-set-2023
2023
533
November 2023
108938
open
Cadamuro, F., Ferrario, M., Akbari, R., Antonini, C., Nicotra, F., Russo, L. (2023). Tyrosine glucosylation of collagen films exploiting Horseradish Peroxidase (HRP). CARBOHYDRATE RESEARCH, 533(November 2023) [10.1016/j.carres.2023.108938].
File in questo prodotto:
File Dimensione Formato  
Cadamuro-2023-Carbohydrate Res-VoR.pdf

accesso aperto

Descrizione: Research Article
Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Creative Commons
Dimensione 3.78 MB
Formato Adobe PDF
3.78 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/441082
Citazioni
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
Social impact