Achieving rigorous control over the procedures aiming at modifying surfaces by selective and covalent anchoring of bioactive molecules is a mandatory step in view of the realistic applicability of bioengineered materials in the field of tissue engineering, biosensing, and nanomedicine. In this context, we report here a proof-of-concept study carried out on a self-assembling peptide (SAP) functionalized with cysteine (Cys), as to ideally grant molecule grafting to gold surfaces. The effectiveness of the surface functionalization in a monolayer regime and the molecular stability of SAP-Cys were probed by X-ray photoelectron spectroscopy; the highly ordered self-organization attained by the grafting molecules was assessed by means of angular-dependent near edge X-ray absorption spectroscopy studies. This study opens wide perspectives for efficient chemical modification of surfaces with biomolecules to include bioactive motifs and/or to add nanometric fibrous patterns.

Secchi, V., Franchi, S., Santi, M., Dettin, M., Zamuner, A., Iucci, G., et al. (2018). Self-Assembling Behavior of Cysteine-Modified Oligopeptides: An XPS and NEXAFS Study. JOURNAL OF PHYSICAL CHEMISTRY. C, 122(11), 6236-6239 [10.1021/acs.jpcc.8b00794].

Self-Assembling Behavior of Cysteine-Modified Oligopeptides: An XPS and NEXAFS Study

Secchi V.;
2018

Abstract

Achieving rigorous control over the procedures aiming at modifying surfaces by selective and covalent anchoring of bioactive molecules is a mandatory step in view of the realistic applicability of bioengineered materials in the field of tissue engineering, biosensing, and nanomedicine. In this context, we report here a proof-of-concept study carried out on a self-assembling peptide (SAP) functionalized with cysteine (Cys), as to ideally grant molecule grafting to gold surfaces. The effectiveness of the surface functionalization in a monolayer regime and the molecular stability of SAP-Cys were probed by X-ray photoelectron spectroscopy; the highly ordered self-organization attained by the grafting molecules was assessed by means of angular-dependent near edge X-ray absorption spectroscopy studies. This study opens wide perspectives for efficient chemical modification of surfaces with biomolecules to include bioactive motifs and/or to add nanometric fibrous patterns.
Articolo in rivista - Articolo scientifico
Amino acids; Chemical modification; Medical nanotechnology; Molecules; Peptides; Tissue engineering; X ray absorption spectroscopy
English
2018
122
11
6236
6239
reserved
Secchi, V., Franchi, S., Santi, M., Dettin, M., Zamuner, A., Iucci, G., et al. (2018). Self-Assembling Behavior of Cysteine-Modified Oligopeptides: An XPS and NEXAFS Study. JOURNAL OF PHYSICAL CHEMISTRY. C, 122(11), 6236-6239 [10.1021/acs.jpcc.8b00794].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/436179
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