A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Colombo, M., Mazzucchelli, S., Montenegro, J., Galbiati, E., Corsi, F., Parak, W., et al. (2012). Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion. SMALL, 8(10), 1492-1497 [10.1002/smll.201102284].

Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion

COLOMBO, MIRIAM;MAZZUCCHELLI, SERENA;GALBIATI, ELISABETTA;PROSPERI, DAVIDE
2012

Abstract

A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Articolo in rivista - Articolo scientifico
BREAST-CANCER CELLS; MAGNETIC NANOPARTICLES; IN-VIVO; SELECTIVE IMMOBILIZATION; COVALENT; TECHNOLOGY; EXPRESSION; ANTIBODIES; THERAPY; ACID
English
2012
8
10
1492
1497
none
Colombo, M., Mazzucchelli, S., Montenegro, J., Galbiati, E., Corsi, F., Parak, W., et al. (2012). Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion. SMALL, 8(10), 1492-1497 [10.1002/smll.201102284].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/43317
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