A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Colombo, M., Mazzucchelli, S., Montenegro, J., Galbiati, E., Corsi, F., Parak, W., et al. (2012). Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion. SMALL, 8(10), 1492-1497 [10.1002/smll.201102284].
Citazione: | Colombo, M., Mazzucchelli, S., Montenegro, J., Galbiati, E., Corsi, F., Parak, W., et al. (2012). Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion. SMALL, 8(10), 1492-1497 [10.1002/smll.201102284]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | Si | |
Titolo: | Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion | |
Autori: | Colombo, M; Mazzucchelli, S; Montenegro, J; Galbiati, E; Corsi, F; Parak, W; Prosperi, D | |
Autori: | ||
Data di pubblicazione: | 2012 | |
Lingua: | English | |
Rivista: | SMALL | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/smll.201102284 | |
Appare nelle tipologie: | 01 - Articolo su rivista |