Aim: To realize and characterize a new generation of keratin-coated gold nanoparticles (Ker-AuNPs) as highly efficient photosensitive nanosized therapeutics for plasmonic photothermal (PPT) therapy. Materials & methods: The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ζ-potential, UV-Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, transmission electron microscopy and high-resolution thermography. In vitro experiments are performed on a human glioblastoma cell line (i.e., U87-MG), using viability assays, transmission electron microscopy, fluorescence microscopy, cytometric analyses and PPT experiments. Results: Experiments confirm the excellent biocompatibility of Ker-AuNPs, their efficient cellular uptake and localized photothermal heating capabilities. Conclusion: The reported structural and functional properties pointed out these Ker-AuNPs as a promising new tool in the field of biocompatible photothermal agents for PPT treatments against cancer-related diseases. This work involves the synthesis and application of nanosized keratin-coated gold particles; a protein extracted from wool. Their effect against a specific type of brain cancer, glioblastoma, is investigated. Gold nanoparticles have been the subject of a lot of interest recently due to their ability to convert light energy into heat energy. When the keratin-coated nanoparticles were up taken into cancerous cells and exposed to light from a laser, they heated up and killed the cancer cells. The next step for this research is to conduct more detailed studies in animals, hopefully leading to a new generation of nanosized therapeutics in the fight against cancer.

Guglielmelli, A., Rosa, P., Contardi, M., Prato, M., Mangino, G., Miglietta, S., et al. (2021). Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme. NANOMEDICINE, 16(2), 121-138 [10.2217/nnm-2020-0349].

Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme

Contardi M.;
2021

Abstract

Aim: To realize and characterize a new generation of keratin-coated gold nanoparticles (Ker-AuNPs) as highly efficient photosensitive nanosized therapeutics for plasmonic photothermal (PPT) therapy. Materials & methods: The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ζ-potential, UV-Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, transmission electron microscopy and high-resolution thermography. In vitro experiments are performed on a human glioblastoma cell line (i.e., U87-MG), using viability assays, transmission electron microscopy, fluorescence microscopy, cytometric analyses and PPT experiments. Results: Experiments confirm the excellent biocompatibility of Ker-AuNPs, their efficient cellular uptake and localized photothermal heating capabilities. Conclusion: The reported structural and functional properties pointed out these Ker-AuNPs as a promising new tool in the field of biocompatible photothermal agents for PPT treatments against cancer-related diseases. This work involves the synthesis and application of nanosized keratin-coated gold particles; a protein extracted from wool. Their effect against a specific type of brain cancer, glioblastoma, is investigated. Gold nanoparticles have been the subject of a lot of interest recently due to their ability to convert light energy into heat energy. When the keratin-coated nanoparticles were up taken into cancerous cells and exposed to light from a laser, they heated up and killed the cancer cells. The next step for this research is to conduct more detailed studies in animals, hopefully leading to a new generation of nanosized therapeutics in the fight against cancer.
Articolo in rivista - Articolo scientifico
cancer; gold nanoparticles; keratin; optics; thermoplasmonics;
English
2021
16
2
121
138
none
Guglielmelli, A., Rosa, P., Contardi, M., Prato, M., Mangino, G., Miglietta, S., et al. (2021). Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme. NANOMEDICINE, 16(2), 121-138 [10.2217/nnm-2020-0349].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/431980
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