The cold-adapted Pseudomonas fragi lipase (PFL) displays highest activity on short-chain triglyceride substrates and is rapidly inactivated at moderate temperature. Sequence and structure comparison with homologous lipases endowed with different substrate specificity and stability, pointed to three polar residues in the lid region, that were replaced with the amino acids conserved at equivalent positions in the reference lipases. Substitutions at residues T137 and T138 modified the lipase chainlength preference profile, increasing the relative activity towards C8 substrates. Moreover, mutations conferred to PFL higher temperature stability. On the other hand, replacement of the serine at position 141 by glycine destabilized the protein.

Santarossa, G., Gatti Lafranconi, P., Alquati, C., DE GIOIA, L., Alberghina, L., Fantucci, P., et al. (2005). Mutations in the lid region affect chain length specificity and thermostability of a Pseudomonas fragi lipase. FEBS LETTERS, 579(11), 2383-2386 [10.1016/j.febslet.2005.03.037].

Mutations in the lid region affect chain length specificity and thermostability of a Pseudomonas fragi lipase

DE GIOIA, LUCA;ALBERGHINA, LILIA;FANTUCCI, PIERCARLO;LOTTI, MARINA
2005

Abstract

The cold-adapted Pseudomonas fragi lipase (PFL) displays highest activity on short-chain triglyceride substrates and is rapidly inactivated at moderate temperature. Sequence and structure comparison with homologous lipases endowed with different substrate specificity and stability, pointed to three polar residues in the lid region, that were replaced with the amino acids conserved at equivalent positions in the reference lipases. Substitutions at residues T137 and T138 modified the lipase chainlength preference profile, increasing the relative activity towards C8 substrates. Moreover, mutations conferred to PFL higher temperature stability. On the other hand, replacement of the serine at position 141 by glycine destabilized the protein.
Articolo in rivista - Articolo scientifico
Lipase; Chain length selectivity; Thermostability; Pseudomonas fragi
English
2005
579
11
2383
2386
none
Santarossa, G., Gatti Lafranconi, P., Alquati, C., DE GIOIA, L., Alberghina, L., Fantucci, P., et al. (2005). Mutations in the lid region affect chain length specificity and thermostability of a Pseudomonas fragi lipase. FEBS LETTERS, 579(11), 2383-2386 [10.1016/j.febslet.2005.03.037].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/422
Citazioni
  • Scopus 91
  • ???jsp.display-item.citation.isi??? 79
Social impact