MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.

Mainini, V., Bovo, G., Chinello, C., Gianazza, E., Grasso, M., Cattoretti, G., et al. (2013). Detection of high molecular weight proteins by MALDI imaging mass spectrometry. MOLECULAR BIOSYSTEMS, 9(6), 1101-1107 [10.1039/c2mb25296a].

Detection of high molecular weight proteins by MALDI imaging mass spectrometry

MAININI, VERONICA
;
CHINELLO, CLIZIA;GIANAZZA, ERICA;CATTORETTI, GIORGIO;MAGNI, FULVIO
2013

Abstract

MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.
Articolo in rivista - Articolo scientifico
imaging; mass spectrometry; proteomics;
English
20-dic-2012
2013
9
6
1101
1107
none
Mainini, V., Bovo, G., Chinello, C., Gianazza, E., Grasso, M., Cattoretti, G., et al. (2013). Detection of high molecular weight proteins by MALDI imaging mass spectrometry. MOLECULAR BIOSYSTEMS, 9(6), 1101-1107 [10.1039/c2mb25296a].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/41774
Citazioni
  • Scopus 38
  • ???jsp.display-item.citation.isi??? 38
Social impact