Bacterial transcription factors (TFs) are widely studied in Escherichia coli. Yet it remains unclear how individual genes in the underlying pathways of TF machinery operate together during environmental challenge. Here, we address this by applying an unbiased, quantitative synthetic genetic interaction (GI) approach to measure pairwise GIs among all TF genes in E. coli under auxotrophic (rich medium) and prototrophic (minimal medium) static growth conditions. The resulting static and differential GI networks reveal condition-dependent GIs, widespread changes among TF genes in metabolism, and new roles for uncharacterized TFs (yjdC, yneJ, ydiP) as regulators of cell division, putrescine utilization pathway, and cold shock adaptation. Pan-bacterial conservation suggests TF genes with GIs are co-conserved in evolution. Together, our results illuminate the global organization of E. coli TFs, and remodeling of genetic backup systems for TFs under environmental change, which is essential for controlling the bacterial transcriptional regulatory circuits.

Gagarinova, A., Hosseinnia, A., Rahmatbakhsh, M., Istace, Z., Phanse, S., Moutaoufik, M., et al. (2022). Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli. NATURE COMMUNICATIONS, 13(1) [10.1038/s41467-022-31819-x].

Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli

Zilocchi, Mara;
2022

Abstract

Bacterial transcription factors (TFs) are widely studied in Escherichia coli. Yet it remains unclear how individual genes in the underlying pathways of TF machinery operate together during environmental challenge. Here, we address this by applying an unbiased, quantitative synthetic genetic interaction (GI) approach to measure pairwise GIs among all TF genes in E. coli under auxotrophic (rich medium) and prototrophic (minimal medium) static growth conditions. The resulting static and differential GI networks reveal condition-dependent GIs, widespread changes among TF genes in metabolism, and new roles for uncharacterized TFs (yjdC, yneJ, ydiP) as regulators of cell division, putrescine utilization pathway, and cold shock adaptation. Pan-bacterial conservation suggests TF genes with GIs are co-conserved in evolution. Together, our results illuminate the global organization of E. coli TFs, and remodeling of genetic backup systems for TFs under environmental change, which is essential for controlling the bacterial transcriptional regulatory circuits.
Articolo in rivista - Articolo scientifico
Epistasis, Bacterial genetics, Bacterial systems biology
English
14-lug-2022
2022
13
1
4085
none
Gagarinova, A., Hosseinnia, A., Rahmatbakhsh, M., Istace, Z., Phanse, S., Moutaoufik, M., et al. (2022). Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli. NATURE COMMUNICATIONS, 13(1) [10.1038/s41467-022-31819-x].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/417318
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