The piperidine nitroxide TEMPOL induces apoptosis in a number of tumor cell lines through free radical-dependent mechanisms. As mitochondria play a major role in apoptosis as both source and target for free radicals, the present study focuses on mitochondrial effects of TEMPOL in a human promyelocytic leukemic cell line (HL-60). On 24-h exposure to TEMPOL, the following alterations were observed: 1) decrease in both the intracellular and mitochondrial glutathione pools; 2) impairment of oxidative phosphorylation; and 3) decrease in mitochondrial membrane potential. In addition, TEMPOL was found to specifically target complex I of the respiratory chain, with minor effects on complexes II and IV, suggesting that mitochondrial effects might play a role in TEMPOL-induced oxidative stress and apoptosis, and that TEMPOL might sensitize tumor cells to the pro-apoptotic effects of cytotoxic agents.

Monti, E., Supino, R., Colleoni, M., Costa, B., Ravizza, R., Gariboldi, M. (2001). Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(2), 271-276 [10.1002/jcb.1160].

Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells

COSTA, BARBARA SIMONA;
2001

Abstract

The piperidine nitroxide TEMPOL induces apoptosis in a number of tumor cell lines through free radical-dependent mechanisms. As mitochondria play a major role in apoptosis as both source and target for free radicals, the present study focuses on mitochondrial effects of TEMPOL in a human promyelocytic leukemic cell line (HL-60). On 24-h exposure to TEMPOL, the following alterations were observed: 1) decrease in both the intracellular and mitochondrial glutathione pools; 2) impairment of oxidative phosphorylation; and 3) decrease in mitochondrial membrane potential. In addition, TEMPOL was found to specifically target complex I of the respiratory chain, with minor effects on complexes II and IV, suggesting that mitochondrial effects might play a role in TEMPOL-induced oxidative stress and apoptosis, and that TEMPOL might sensitize tumor cells to the pro-apoptotic effects of cytotoxic agents.
Articolo in rivista - Articolo scientifico
apoptosis; mitochondria; glutathione; oxidative stress; TEMPOL
English
2001
82
2
271
276
none
Monti, E., Supino, R., Colleoni, M., Costa, B., Ravizza, R., Gariboldi, M. (2001). Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(2), 271-276 [10.1002/jcb.1160].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/4160
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