The piperidine nitroxide TEMPOL induces apoptosis in a number of tumor cell lines through free radical-dependent mechanisms. As mitochondria play a major role in apoptosis as both source and target for free radicals, the present study focuses on mitochondrial effects of TEMPOL in a human promyelocytic leukemic cell line (HL-60). On 24-h exposure to TEMPOL, the following alterations were observed: 1) decrease in both the intracellular and mitochondrial glutathione pools; 2) impairment of oxidative phosphorylation; and 3) decrease in mitochondrial membrane potential. In addition, TEMPOL was found to specifically target complex I of the respiratory chain, with minor effects on complexes II and IV, suggesting that mitochondrial effects might play a role in TEMPOL-induced oxidative stress and apoptosis, and that TEMPOL might sensitize tumor cells to the pro-apoptotic effects of cytotoxic agents.

Monti, E., Supino, R., Colleoni, M., Costa, B., Ravizza, R., & Gariboldi, M. (2001). Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(2), 271-276 [10.1002/jcb.1160].

Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells

COSTA, BARBARA SIMONA;
2001

Abstract

The piperidine nitroxide TEMPOL induces apoptosis in a number of tumor cell lines through free radical-dependent mechanisms. As mitochondria play a major role in apoptosis as both source and target for free radicals, the present study focuses on mitochondrial effects of TEMPOL in a human promyelocytic leukemic cell line (HL-60). On 24-h exposure to TEMPOL, the following alterations were observed: 1) decrease in both the intracellular and mitochondrial glutathione pools; 2) impairment of oxidative phosphorylation; and 3) decrease in mitochondrial membrane potential. In addition, TEMPOL was found to specifically target complex I of the respiratory chain, with minor effects on complexes II and IV, suggesting that mitochondrial effects might play a role in TEMPOL-induced oxidative stress and apoptosis, and that TEMPOL might sensitize tumor cells to the pro-apoptotic effects of cytotoxic agents.
Articolo in rivista - Articolo scientifico
apoptosis; mitochondria; glutathione; oxidative stress; TEMPOL
English
Monti, E., Supino, R., Colleoni, M., Costa, B., Ravizza, R., & Gariboldi, M. (2001). Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(2), 271-276 [10.1002/jcb.1160].
Monti, E; Supino, R; Colleoni, M; Costa, B; Ravizza, R; Gariboldi, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/4160
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