The piperidine nitroxide TEMPOL induces apoptosis in a number of tumor cell lines through free radical-dependent mechanisms. As mitochondria play a major role in apoptosis as both source and target for free radicals, the present study focuses on mitochondrial effects of TEMPOL in a human promyelocytic leukemic cell line (HL-60). On 24-h exposure to TEMPOL, the following alterations were observed: 1) decrease in both the intracellular and mitochondrial glutathione pools; 2) impairment of oxidative phosphorylation; and 3) decrease in mitochondrial membrane potential. In addition, TEMPOL was found to specifically target complex I of the respiratory chain, with minor effects on complexes II and IV, suggesting that mitochondrial effects might play a role in TEMPOL-induced oxidative stress and apoptosis, and that TEMPOL might sensitize tumor cells to the pro-apoptotic effects of cytotoxic agents.
Monti, E., Supino, R., Colleoni, M., Costa, B., Ravizza, R., & Gariboldi, M.B. (2001). Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(2), 271-276.
Citazione: | Monti, E., Supino, R., Colleoni, M., Costa, B., Ravizza, R., & Gariboldi, M.B. (2001). Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(2), 271-276. |
Tipo: | Articolo in rivista - Articolo scientifico |
Carattere della pubblicazione: | Scientifica |
Titolo: | Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells |
Autori: | Monti, E; Supino, R; Colleoni, M; Costa, B; Ravizza, R; Gariboldi, MB |
Autori: | |
Data di pubblicazione: | 2001 |
Lingua: | English |
Rivista: | JOURNAL OF CELLULAR BIOCHEMISTRY |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/jcb.1160 |
Appare nelle tipologie: | 01 - Articolo su rivista |