Introduction In central nervous system neurodegenerative disorders, stem cell-based therapies should be considered as a promising therapeutic approach. The safe use of human Neural Stem Cells (hNSCs) for the treatment of several neurological diseases is currently under evaluation of phase I/II clinical trials. Clinical application of hNSCs require the development of GMP standardized protocols capable of generating high quantities of reproducible and well characterized stem cells bearing stable functional and genetic properties. Aim The aim of this study was to evaluate possible instabilities or modifications of the microsatellite loci in different culture passages because high culture passages represent an in vitro replicative stress leading to senescence. Experimental method: The hNSCs were characterized at different culture time points, from passage 2 to passage 25, by genetic typing at ten microsatellite loci. Conclusion We showed that genetic stability at microsatellite loci is maintained by the cells even at high passages adding a further demonstration of the safety of our hNSCs GMP culture method.

Grespi, V., Caprera, C., Ricciolini, C., Bicchi, I., Muzi, G., Corsi, M., et al. (2022). Human neural stem cells drug product: Microsatellite instability analysis. PLOS ONE, 17(8) [10.1371/journal.pone.0273679].

Human neural stem cells drug product: Microsatellite instability analysis

Vescovi A. L.;
2022

Abstract

Introduction In central nervous system neurodegenerative disorders, stem cell-based therapies should be considered as a promising therapeutic approach. The safe use of human Neural Stem Cells (hNSCs) for the treatment of several neurological diseases is currently under evaluation of phase I/II clinical trials. Clinical application of hNSCs require the development of GMP standardized protocols capable of generating high quantities of reproducible and well characterized stem cells bearing stable functional and genetic properties. Aim The aim of this study was to evaluate possible instabilities or modifications of the microsatellite loci in different culture passages because high culture passages represent an in vitro replicative stress leading to senescence. Experimental method: The hNSCs were characterized at different culture time points, from passage 2 to passage 25, by genetic typing at ten microsatellite loci. Conclusion We showed that genetic stability at microsatellite loci is maintained by the cells even at high passages adding a further demonstration of the safety of our hNSCs GMP culture method.
Articolo in rivista - Articolo scientifico
Cell Differentiation; Humans; Microsatellite Instability; Neural Stem Cells; Stem Cell Transplantation;
English
30-ago-2022
2022
17
8
e0273679
open
Grespi, V., Caprera, C., Ricciolini, C., Bicchi, I., Muzi, G., Corsi, M., et al. (2022). Human neural stem cells drug product: Microsatellite instability analysis. PLOS ONE, 17(8) [10.1371/journal.pone.0273679].
File in questo prodotto:
File Dimensione Formato  
10281-412816_VoR.pdf

accesso aperto

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Creative Commons
Dimensione 322.05 kB
Formato Adobe PDF
322.05 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/412816
Citazioni
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
Social impact