Animal models currently used to test the efficacy and safety of cell therapies, mainly murine models, have limitations as molecular, cellular, and physiological mechanisms are often inherently different between species, especially in the brain. Therefore, for clinical translation of cell-based medicinal products, the development of alternative models based on human neural cells may be crucial. We have developed an in vitro model of transplantation into human brain organoids to study the potential of neural stem cells as cell therapeutics and compared these data with standard xenograft studies in the brain of immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. Neural stem cells showed similar differentiation and proliferation potentials in both human brain organoids and mouse brains. Our results suggest that brain organoids can be informative in the evaluation of cell therapies, helping to reduce the number of animals used for regulatory studies.

Garcia-Delgado, A., Campos-Cuerva, R., Rosell-Valle, C., Martin-Lopez, M., Casado, C., Ferrari, D., et al. (2022). Brain Organoids to Evaluate Cellular Therapies. ANIMALS, 12(22), 1-13 [10.3390/ani12223150].

Brain Organoids to Evaluate Cellular Therapies

Ferrari D.;
2022

Abstract

Animal models currently used to test the efficacy and safety of cell therapies, mainly murine models, have limitations as molecular, cellular, and physiological mechanisms are often inherently different between species, especially in the brain. Therefore, for clinical translation of cell-based medicinal products, the development of alternative models based on human neural cells may be crucial. We have developed an in vitro model of transplantation into human brain organoids to study the potential of neural stem cells as cell therapeutics and compared these data with standard xenograft studies in the brain of immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. Neural stem cells showed similar differentiation and proliferation potentials in both human brain organoids and mouse brains. Our results suggest that brain organoids can be informative in the evaluation of cell therapies, helping to reduce the number of animals used for regulatory studies.
Articolo in rivista - Articolo scientifico
3 Rs; brain organoids; cell therapy; neural progenitors; neural stem cells; reduction; translation;
English
15-nov-2022
2022
12
22
1
13
3150
open
Garcia-Delgado, A., Campos-Cuerva, R., Rosell-Valle, C., Martin-Lopez, M., Casado, C., Ferrari, D., et al. (2022). Brain Organoids to Evaluate Cellular Therapies. ANIMALS, 12(22), 1-13 [10.3390/ani12223150].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/412744
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