Waldenström Macroglobulinemia (WM) is a B-cell lymphoma characterized by the precursor condition IgM monoclonal gammopathies of undetermined significance (IgM MGUS). We performed a gene expression profiling study to compare the transcriptome signatures of bone marrow (BM) B-cells and plasma cells of 36 WM patients, 13 IgM MGUS cases, and 7 healthy subjects used as controls (CTRLs) by Affymetrix microarray. We determined 2038 differentially expressed genes (DEGs) in CD19+ cells and 29 DEGs genes in CD138+ cells, respectively. The DEGs identified in B-cells were associated with KEGG pathways, mainly involved in hematopoietic cell lineage antigens, cell adhesion/focal adhesion/transmembrane proteins, adherens junctions, Wnt-signaling pathway, BCR-signaling pathway, calcium signaling pathway, complement/coagulation cascade, platelet activation, cytokine-cytokine receptor interactions, and signaling pathways responsible for cell cycle, apoptosis, proliferation and survival. In conclusion, we showed the deregulation of groups of genes belonging to KEGG pathways in the comparison among WM vs. IgM MGUS vs. CTRLs in B-cells. Interestingly, a small set of genes in B-cells displayed a common transcriptome expression profile between WM and IgM MGUS compared to CTRLs, suggesting its possible role in the risk of transformation of IgM MGUS to WM.

Alessandra, T., Barbara Di, C., Luca Emanuele, B., Livia, L., Antonino, G., Alessandra, T., et al. (2021). Identification of a candidate gene set signature for the risk of progression in igm mgus to smoldering/symptomatic waldenström macroglobulinemia (Wm) by a comparative transcriptome analysis of b cells and plasma cells. CANCERS, 13(8) [10.3390/cancers13081837].

Identification of a candidate gene set signature for the risk of progression in igm mgus to smoldering/symptomatic waldenström macroglobulinemia (Wm) by a comparative transcriptome analysis of b cells and plasma cells

Cairoli Roberto
2021

Abstract

Waldenström Macroglobulinemia (WM) is a B-cell lymphoma characterized by the precursor condition IgM monoclonal gammopathies of undetermined significance (IgM MGUS). We performed a gene expression profiling study to compare the transcriptome signatures of bone marrow (BM) B-cells and plasma cells of 36 WM patients, 13 IgM MGUS cases, and 7 healthy subjects used as controls (CTRLs) by Affymetrix microarray. We determined 2038 differentially expressed genes (DEGs) in CD19+ cells and 29 DEGs genes in CD138+ cells, respectively. The DEGs identified in B-cells were associated with KEGG pathways, mainly involved in hematopoietic cell lineage antigens, cell adhesion/focal adhesion/transmembrane proteins, adherens junctions, Wnt-signaling pathway, BCR-signaling pathway, calcium signaling pathway, complement/coagulation cascade, platelet activation, cytokine-cytokine receptor interactions, and signaling pathways responsible for cell cycle, apoptosis, proliferation and survival. In conclusion, we showed the deregulation of groups of genes belonging to KEGG pathways in the comparison among WM vs. IgM MGUS vs. CTRLs in B-cells. Interestingly, a small set of genes in B-cells displayed a common transcriptome expression profile between WM and IgM MGUS compared to CTRLs, suggesting its possible role in the risk of transformation of IgM MGUS to WM.
Articolo in rivista - Articolo scientifico
DEGs (differentially expressed genes); Gene expression profiling; IgM monoclonal gammopathies of undetermined significance; KEGG signaling pathways; Waldenström Macroglobulinemia
English
2021
13
8
1837
open
Alessandra, T., Barbara Di, C., Luca Emanuele, B., Livia, L., Antonino, G., Alessandra, T., et al. (2021). Identification of a candidate gene set signature for the risk of progression in igm mgus to smoldering/symptomatic waldenström macroglobulinemia (Wm) by a comparative transcriptome analysis of b cells and plasma cells. CANCERS, 13(8) [10.3390/cancers13081837].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/403475
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