The waste stream of low-grade wool is an underutilized source of keratin-rich materials with appropriate methods for upcycling into high value-added products still being an open challenge. In the present work, keratins were precipitated from their water solution to produce hierarchical keratin particles via isoelectric precipitation. Matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry analysis (MALDI-TOF/TOF MS/MS) showed the presence of the amino acid sequence leucine-aspartic acid-valine (LDV) in the extracted keratin. This well-known cell adhesion motif is recognized by the cell adhesion molecule α4β1 integrin. We showed that keratin particles had this tripeptide exposed on the surface and that it could be leveraged, via patterns obtained with microcontact printing, to support and facilitate dermal fibroblast cell adhesion and direct their growth orientation. The zeta potential, isoelectric point, morphological structures, chemical composition, and biocompatibility of keratin particles and the influence of the surfactant sodium dodecyl sulfate (SDS) were investigated. An appropriate ink for microcontact printing of the keratin particles was developed and micron-sized patterns were obtained. Cells adhered preferentially to the patterns, showing how this strategy could be used to functionalize biointerfaces.

Trojanowska, D., Suarato, G., Braccia, C., Armirotti, A., Fiorentini, F., Athanassiou, A., et al. (2022). Wool Keratin Nanoparticle-Based Micropatterns for Cellular Guidance Applications. ACS APPLIED NANO MATERIALS, 5(10), 15272-15287 [10.1021/acsanm.2c03116].

Wool Keratin Nanoparticle-Based Micropatterns for Cellular Guidance Applications

Trojanowska D. J.
Primo
;
2022

Abstract

The waste stream of low-grade wool is an underutilized source of keratin-rich materials with appropriate methods for upcycling into high value-added products still being an open challenge. In the present work, keratins were precipitated from their water solution to produce hierarchical keratin particles via isoelectric precipitation. Matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry analysis (MALDI-TOF/TOF MS/MS) showed the presence of the amino acid sequence leucine-aspartic acid-valine (LDV) in the extracted keratin. This well-known cell adhesion motif is recognized by the cell adhesion molecule α4β1 integrin. We showed that keratin particles had this tripeptide exposed on the surface and that it could be leveraged, via patterns obtained with microcontact printing, to support and facilitate dermal fibroblast cell adhesion and direct their growth orientation. The zeta potential, isoelectric point, morphological structures, chemical composition, and biocompatibility of keratin particles and the influence of the surfactant sodium dodecyl sulfate (SDS) were investigated. An appropriate ink for microcontact printing of the keratin particles was developed and micron-sized patterns were obtained. Cells adhered preferentially to the patterns, showing how this strategy could be used to functionalize biointerfaces.
Articolo in rivista - Articolo scientifico
biointerfaces; keratin; laser desorption/ionization; leveraged; molecule;
English
4-ott-2022
2022
5
10
15272
15287
open
Trojanowska, D., Suarato, G., Braccia, C., Armirotti, A., Fiorentini, F., Athanassiou, A., et al. (2022). Wool Keratin Nanoparticle-Based Micropatterns for Cellular Guidance Applications. ACS APPLIED NANO MATERIALS, 5(10), 15272-15287 [10.1021/acsanm.2c03116].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/401095
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