Decellularized matrices are steadily gaining popularity to study the biology of cells and tissues, as they represent a biomimetic environment in which cells can recapitulate certain behaviours that share similarities with those observed in vivo. Basically, biochemistry, microstructure and mechanics of the decellularized matrices are the most valuable properties that differentiate these culturing systems from conventional bidimensional models. Several procedures to decellularize tissues have been proposed so far, with the common aim to preserve the tissue chemical/physical properties of the original tissue. However, these processes are complex, time-consuming and expensive. In this work, we propose a cost-effective, easy-to-produce decellularized dermal matrix, derived from animal skin. The chemical/physical processes to obtain the matrices proved to not alter matrix structure and did not induce cytotoxicity issues. To test the validity of the decellularized matrices as a model to study the behaviour of tumour cells in vitro, we performed microstructural and mechanical investigations as well as cell proliferation assays. In particular, three different tumour cell lines were used, which proliferated and invaded the matrix with no additional treatments. Decellularized skin scaffold, presented in this work, could be a strong competitor for conventional 3D systems like synthetic porous scaffolds or hydrogels.

Brancato, V., Ventre, M., Imparato, G., Urciuolo, F., Meo, C., Netti, P. (2018). A straightforward method to produce decellularized dermis-based matrices for tumour cell cultures. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 12(1), 71-81 [10.1002/term.2350].

A straightforward method to produce decellularized dermis-based matrices for tumour cell cultures

Brancato, V;
2018

Abstract

Decellularized matrices are steadily gaining popularity to study the biology of cells and tissues, as they represent a biomimetic environment in which cells can recapitulate certain behaviours that share similarities with those observed in vivo. Basically, biochemistry, microstructure and mechanics of the decellularized matrices are the most valuable properties that differentiate these culturing systems from conventional bidimensional models. Several procedures to decellularize tissues have been proposed so far, with the common aim to preserve the tissue chemical/physical properties of the original tissue. However, these processes are complex, time-consuming and expensive. In this work, we propose a cost-effective, easy-to-produce decellularized dermal matrix, derived from animal skin. The chemical/physical processes to obtain the matrices proved to not alter matrix structure and did not induce cytotoxicity issues. To test the validity of the decellularized matrices as a model to study the behaviour of tumour cells in vitro, we performed microstructural and mechanical investigations as well as cell proliferation assays. In particular, three different tumour cell lines were used, which proliferated and invaded the matrix with no additional treatments. Decellularized skin scaffold, presented in this work, could be a strong competitor for conventional 3D systems like synthetic porous scaffolds or hydrogels.
Articolo in rivista - Articolo scientifico
collagen networks; collagen stiffness; decellularized dermis; dehydration process; tumour cell proliferation; tumour cells;
English
71
81
11
Brancato, V., Ventre, M., Imparato, G., Urciuolo, F., Meo, C., Netti, P. (2018). A straightforward method to produce decellularized dermis-based matrices for tumour cell cultures. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 12(1), 71-81 [10.1002/term.2350].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/401013
Citazioni
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 8
Social impact