The cytoskeleton is a network of proteins which structurally and dynamically organise the cytoplasm of living cells. Microtubules are among its constituents. Morphological alterations of microtubules are related to functional impairment. Therefore cytoskeletal morphology is a valuable indicator of cell injury and functionality. This paper focuses on the comparison between normal and altered cytoskeletal microtubules by means of image analysis and classification with the aim of replacing visual assessment. Morphology has been quantified by the extraction of some descriptors yielded by spatial differentiation, fractal analysis and Fourier analysis followed by non-linear filtering. The principal component analysis of these descriptors has led to image recognition and has been applied to hepatocytes and fibroblasts exposed to some xenobiotics. In the case of hepatocytes, images have been ranked according to the severity of cytoskeletal damage, a dose-response relation has been derived from the regression of the first principal component and the percentage of structural recovery after exposure has been estimated.

Fumarola, L., Urani, C., & Crosta, G. (2005). Quantitative kinetics of damage and recovery of cytoskeletal structure by means of image analysis. TOXICOLOGY IN VITRO, 19(7), 935-941 [10.1016/j.tiv.2005.06.012].

Quantitative kinetics of damage and recovery of cytoskeletal structure by means of image analysis

URANI, CHIARA;CROSTA, GIOVANNI FRANCO FILIPPO
2005-08-02

Abstract

The cytoskeleton is a network of proteins which structurally and dynamically organise the cytoplasm of living cells. Microtubules are among its constituents. Morphological alterations of microtubules are related to functional impairment. Therefore cytoskeletal morphology is a valuable indicator of cell injury and functionality. This paper focuses on the comparison between normal and altered cytoskeletal microtubules by means of image analysis and classification with the aim of replacing visual assessment. Morphology has been quantified by the extraction of some descriptors yielded by spatial differentiation, fractal analysis and Fourier analysis followed by non-linear filtering. The principal component analysis of these descriptors has led to image recognition and has been applied to hepatocytes and fibroblasts exposed to some xenobiotics. In the case of hepatocytes, images have been ranked according to the severity of cytoskeletal damage, a dose-response relation has been derived from the regression of the first principal component and the percentage of structural recovery after exposure has been estimated.
Articolo in rivista - Articolo scientifico
FRACTAL ANALYSIS; HEPATOCYTES; TARGETS; IMAGE CLASSIFICATION; CELLS
English
Fumarola, L., Urani, C., & Crosta, G. (2005). Quantitative kinetics of damage and recovery of cytoskeletal structure by means of image analysis. TOXICOLOGY IN VITRO, 19(7), 935-941 [10.1016/j.tiv.2005.06.012].
Fumarola, L; Urani, C; Crosta, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/4005
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