The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration.

Rotem, R., Bertolini, J., Salvioni, L., Barbieri, L., Rizzuto, M., Tinelli, V., et al. (2023). Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2. NANOMEDICINE, 47(January 2023) [10.1016/j.nano.2022.102626].

Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2

Rotem, R
Co-primo
;
Bertolini, JA
Co-primo
;
Salvioni, L;Barbieri, L;Rizzuto, MA;Colombo, M;Prosperi, D
2023

Abstract

The delivery of therapeutics across the cell membrane and into the cytoplasm is a major challenge that limits the development of new therapies. This challenge is compounded by the lack of a general assay for cytosolic delivery. Here we develop this assay based on the pro-fluorophore CrAsH-EDT2, and provide cytosolic penetration results for a variety of drug delivery agents (polyethyleneimine, poly-arginine, Ferritin, poly [maleic anhydride-alt-isobutene] grafted with dodecylamine, and cationic liposomes) into HeLa and T98G cells. Our results show that this method can be widely applicable to different cells and drug delivery agents, and yield statistically robust results. We later use this method to optimize and improve a model drug delivery agent's (Ferritin) cytosolic penetration.
Articolo in rivista - Articolo scientifico
Cell penetration; Drug delivery; FlAsH-EDT2; Flow cytometry; Quantification method;
English
7-nov-2022
2023
47
January 2023
102626
open
Rotem, R., Bertolini, J., Salvioni, L., Barbieri, L., Rizzuto, M., Tinelli, V., et al. (2023). Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2. NANOMEDICINE, 47(January 2023) [10.1016/j.nano.2022.102626].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/400072
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