Faithful chromosome segregation is mandatory for cell and organismal viability. Kinetochores, large protein assemblies embedded in centromeric chromatin, establish a mechanical link between chromosomes and spindle microtubules. The KMN network, a conserved 10-subunit kinetochore complex, harbors the microtubule-binding interface. RWD domains in the KMN subunits Spc24 and Spc25 mediate kinetochore targeting of the microtubule-binding subunits by interacting with the Mis12 complex, a KMN subcomplex that tethers directly onto the underlying chromatin layer. Here, we show that Knl1, a KMN subunit involved in mitotic checkpoint signaling, also contains RWD domains that bind the Mis12 complex and that mediate kinetochore targeting of Knl1. By reporting the first 3D electron microscopy structure of the KMN network, we provide a comprehensive framework to interpret how interactions of RWD-containing proteins with the Mis12 complex shape KMN network topology. Our observations unveil a regular pattern in the construction of the outer kinetochore.

Petrovic, A., Mosalaganti, S., Keller, J., Mattiuzzo, M., Overlack, K., Krenn, V., et al. (2014). Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization. MOLECULAR CELL, 53(4), 591-605 [10.1016/j.molcel.2014.01.019].

Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization

Krenn, Veronica;
2014

Abstract

Faithful chromosome segregation is mandatory for cell and organismal viability. Kinetochores, large protein assemblies embedded in centromeric chromatin, establish a mechanical link between chromosomes and spindle microtubules. The KMN network, a conserved 10-subunit kinetochore complex, harbors the microtubule-binding interface. RWD domains in the KMN subunits Spc24 and Spc25 mediate kinetochore targeting of the microtubule-binding subunits by interacting with the Mis12 complex, a KMN subcomplex that tethers directly onto the underlying chromatin layer. Here, we show that Knl1, a KMN subunit involved in mitotic checkpoint signaling, also contains RWD domains that bind the Mis12 complex and that mediate kinetochore targeting of Knl1. By reporting the first 3D electron microscopy structure of the KMN network, we provide a comprehensive framework to interpret how interactions of RWD-containing proteins with the Mis12 complex shape KMN network topology. Our observations unveil a regular pattern in the construction of the outer kinetochore.
Articolo in rivista - Articolo scientifico
Amino Acid Sequence; Centromere; Chromosome Segregation; Crystallography, X-Ray; Escherichia coli; HeLa Cells; Humans; Kinetochores; M Phase Cell Cycle Checkpoints; Microscopy, Electron; Microtubule-Associated Proteins; Microtubules; Mitosis; Models, Molecular; Molecular Sequence Data; Plasmids; Protein Conformation; Protein Structure, Tertiary; Sequence Homology, Amino Acid
English
2014
53
4
591
605
open
Petrovic, A., Mosalaganti, S., Keller, J., Mattiuzzo, M., Overlack, K., Krenn, V., et al. (2014). Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization. MOLECULAR CELL, 53(4), 591-605 [10.1016/j.molcel.2014.01.019].
File in questo prodotto:
File Dimensione Formato  
Petrovic-2014-Molecular Cell-VoR.pdf

accesso aperto

Descrizione: Article
Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 3.27 MB
Formato Adobe PDF
3.27 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/397721
Citazioni
  • Scopus 97
  • ???jsp.display-item.citation.isi??? 98
Social impact