Background The KMN network, a ten-subunit protein complex, mediates the interaction of kinetochores with spindle microtubules and recruits spindle assembly checkpoint (SAC) constituents to halt cells in mitosis until attainment of sister chromatid biorientation. Two types of motifs in the KMN subunit Knl1 interact with SAC proteins. Lys-Ile (KI) motifs, found in vertebrates, interact with the TPR motifs of Bub1 and BubR1. Met-Glu-Leu-Thr (MELT) repeats, ubiquitous in evolution, recruit the Bub3/Bub1 complex in a phosphorylation- dependent manner. The exact contributions of KI and MELT motifs to SAC signaling and chromosome alignment are unclear. Results We report here that KI motifs cooperate strongly with the neighboring single MELT motif in the N-terminal 250 residues (Knl11-250) of human Knl1 to seed a comprehensive assembly of SAC proteins. In cells depleted of endogenous Knl1, kinetochore-targeted Knl11-250 suffices to restore SAC and chromosome alignment. Individual MELT repeats outside of Knl11-250, which lack flanking KI motifs, establish qualitatively similar sets of interactions, but less efficiently. Conclusions MELT sequences on Knl1 emerge from our analysis as the platforms on which SAC complexes become assembled. Our results show that KI motifs are enhancers of MELT function in assembling SAC signaling complexes, and that they might have evolved to limit the expansion of MELT motifs by providing a more robust mechanism of SAC signaling around a single MELT. We shed light on the mechanism of Bub1 and BubR1 recruitment and identify crucial questions for future studies.

Krenn, V., Overlack, K., Primorac, I., van Gerwen, S., Musacchio, A. (2014). KI motifs of human Knl1 enhance assembly of comprehensive spindle checkpoint complexes around MELT repeats. CURRENT BIOLOGY, 24(1), 29-39 [10.1016/j.cub.2013.11.046].

KI motifs of human Knl1 enhance assembly of comprehensive spindle checkpoint complexes around MELT repeats

Krenn, Veronica;
2014

Abstract

Background The KMN network, a ten-subunit protein complex, mediates the interaction of kinetochores with spindle microtubules and recruits spindle assembly checkpoint (SAC) constituents to halt cells in mitosis until attainment of sister chromatid biorientation. Two types of motifs in the KMN subunit Knl1 interact with SAC proteins. Lys-Ile (KI) motifs, found in vertebrates, interact with the TPR motifs of Bub1 and BubR1. Met-Glu-Leu-Thr (MELT) repeats, ubiquitous in evolution, recruit the Bub3/Bub1 complex in a phosphorylation- dependent manner. The exact contributions of KI and MELT motifs to SAC signaling and chromosome alignment are unclear. Results We report here that KI motifs cooperate strongly with the neighboring single MELT motif in the N-terminal 250 residues (Knl11-250) of human Knl1 to seed a comprehensive assembly of SAC proteins. In cells depleted of endogenous Knl1, kinetochore-targeted Knl11-250 suffices to restore SAC and chromosome alignment. Individual MELT repeats outside of Knl11-250, which lack flanking KI motifs, establish qualitatively similar sets of interactions, but less efficiently. Conclusions MELT sequences on Knl1 emerge from our analysis as the platforms on which SAC complexes become assembled. Our results show that KI motifs are enhancers of MELT function in assembling SAC signaling complexes, and that they might have evolved to limit the expansion of MELT motifs by providing a more robust mechanism of SAC signaling around a single MELT. We shed light on the mechanism of Bub1 and BubR1 recruitment and identify crucial questions for future studies.
Articolo in rivista - Articolo scientifico
HeLa Cells; Humans; Kinetochores; Microtubule-Associated Proteins; Molecular Sequence Data; Peptides; Protein Binding; Protein Serine-Threonine Kinases; Repetitive Sequences, Amino Acid; Sequence Alignment; Spindle Apparatus
English
2014
24
1
29
39
open
Krenn, V., Overlack, K., Primorac, I., van Gerwen, S., Musacchio, A. (2014). KI motifs of human Knl1 enhance assembly of comprehensive spindle checkpoint complexes around MELT repeats. CURRENT BIOLOGY, 24(1), 29-39 [10.1016/j.cub.2013.11.046].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/397714
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