The cellular uptake of nanoparticles (NPs) represents a critical step in nanomedicine and a crucial point for understanding the interaction of nanomaterials with biological systems. No specific mechanism of uptake has been identified so far, as the NPs are generally incorporated by the cells through one of the few well-known endocytotic mechanisms. Here, an alternative internalization route mediated by microvilli adhesion is demonstrated. This microvillus-mediated adhesion (MMA) has been observed using ceria and magnetite NPs with a dimension of <40 nm functionalized with polyacrylic acid but not using NPs with a neutral or positive functionalization. Such an adhesion was not cell specific, as it was demonstrated in three different cell lines. MMA was also reduced by modifications of the microvillus lipid rafts, obtained by depleting cholesterol and altering synthesis of sphingolipids. We found a direct relationship between MAA, cell cycle, and density of microvilli. The evidence suggests that MMA differs from the commonly described uptake mechanisms and might represent an interesting alternative approach for selective NP delivery.

Sommi, P., Vitali, A., Coniglio, S., Callegari, D., Barbieri, S., Casu, A., et al. (2021). Microvilli Adhesion: An Alternative Route for Nanoparticle Cell Internalization. ACS NANO, 15(10), 15803-15814 [10.1021/acsnano.1c03151].

Microvilli Adhesion: An Alternative Route for Nanoparticle Cell Internalization

Viganò, L.;
2021

Abstract

The cellular uptake of nanoparticles (NPs) represents a critical step in nanomedicine and a crucial point for understanding the interaction of nanomaterials with biological systems. No specific mechanism of uptake has been identified so far, as the NPs are generally incorporated by the cells through one of the few well-known endocytotic mechanisms. Here, an alternative internalization route mediated by microvilli adhesion is demonstrated. This microvillus-mediated adhesion (MMA) has been observed using ceria and magnetite NPs with a dimension of <40 nm functionalized with polyacrylic acid but not using NPs with a neutral or positive functionalization. Such an adhesion was not cell specific, as it was demonstrated in three different cell lines. MMA was also reduced by modifications of the microvillus lipid rafts, obtained by depleting cholesterol and altering synthesis of sphingolipids. We found a direct relationship between MAA, cell cycle, and density of microvilli. The evidence suggests that MMA differs from the commonly described uptake mechanisms and might represent an interesting alternative approach for selective NP delivery.
Articolo in rivista - Articolo scientifico
adhesion/internalization; cell microvilli; CeO2; HeLa; nanoparticles;
English
15803
15814
12
Sommi, P., Vitali, A., Coniglio, S., Callegari, D., Barbieri, S., Casu, A., et al. (2021). Microvilli Adhesion: An Alternative Route for Nanoparticle Cell Internalization. ACS NANO, 15(10), 15803-15814 [10.1021/acsnano.1c03151].
Sommi, P; Vitali, A; Coniglio, S; Callegari, D; Barbieri, S; Casu, A; Falqui, A; Viganò, L; Vigani, B; Ferrari, F; Anselmi-Tamburini, U
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/395175
Citazioni
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 5
Social impact