CALCIUM SULFATE LEADS TO OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED STEM CELLS

The ideal bone graft material would be biocompatible, completely biodegradable, osteoconductive, unexpensive, easy to handle, and able to support the defect area until bone growth is complete. Several bone graft materials are available, but none of them have thus far satisfied all of these requirements. Calcium sulfate is one of the oldest bone graft materials and possesses good effect on acceletaring bone healing and regeneration. To study the mechanism how CaS can induce differentiation and proliferation in ADSCs the expression levels of several bone related genes were analyzed using real time Reverse Transcription-Polymerase Chain Reaction. Our results show that CaS strongly influences the behavior of ADSCs in vitro by enhancing proliferation, differentiation and deposition of matrix by increasing the activity of RUNX2, SP7 and SPP1.