INFLUENCE OF PEPTIDE-15 ON ADIPOSE DERIVED STEM CELLS

A bone substitute graft should facilitate cell adhesion, promote osteoblastic differentiation and enhance matrix. P-15 (Ceramed, Lakewood, CO) is an analog of the cell-binding domain of collagen, a protein involved in regulation of cell adhesion and in osteogenesis. To study how P15 can induce osteoblast differentiation and proliferation in mesenchymal stem cells, the expression levels of bone related genes (RUNX2, SP7, ALPL, SPP1, COL1A1, COL3A1 and FOSL1) and mesenchymal stem cells marker (ENG) were measured in Adipose Derived Stem Cells (DPSCs) and Normal Osteoblast (NO), after 15 and 30 days of treatment. Significantly, differentially expressed genes among ADSCs and NO were ENG, FOSL1, RUNX2, COL3A1, COL1A1 and ALPL in the first 15 days of treatment and SP7, ENG, RUNX2, COL1A1, SPP1 and ALPL after 30 days. The present study demonstrated that P15 influences the behavior of DPSCs in vitro by enhancing proliferation, differentiation and deposition of matrix as demonstrated by the activation of osteoblast related genes FOSL1, SP7 and SPPl.