Objective: The aim of the present study was to evaluate the role of the renal nerves in the regulation of neuronal nitric oxide synthase (nNOS) gene expression in normotensive rats on different sodium balance. Methods: Thirty-six male Sprague-Dawley rats were divided into six experimental groups combining three diets with different NaCl content (normal, 0.4%; low, 0.04%; or high, 4.0%), and bilateral renal denervation or sham denervation. After 7 days of dietary treatment, all rats were sacrificed and plasma renin activity (PRA) measured. The nNOS and renin messenger RNA (mRNA) levels in the renal cortex were determined by semiquantitative polymerase chain reaction. Results: PRA was higher in animals with low sodium diet compared with those with standard diet, while it was lower in animals with high sodium diet. Renal denervation decreased PRA in normal and low sodium groups, while it did not alter the PRA values in the high sodium group. The nNOS gene expression significantly increase...

Pieruzzi, F., Munforti, C., Di Blasio, A., Busca, G., Dadone, V., Zanchetti, A., et al. (2002). Neuronal nitric oxide synthase and renin stimulation by sodium deprivation are dependent on the renal nerves. JOURNAL OF HYPERTENSION, 20(10), 2039-2045 [10.1097/00004872-200210000-00024].

Neuronal nitric oxide synthase and renin stimulation by sodium deprivation are dependent on the renal nerves

PIERUZZI, FEDERICO UMBERTO EMILIO GUGLIE;
2002

Abstract

Objective: The aim of the present study was to evaluate the role of the renal nerves in the regulation of neuronal nitric oxide synthase (nNOS) gene expression in normotensive rats on different sodium balance. Methods: Thirty-six male Sprague-Dawley rats were divided into six experimental groups combining three diets with different NaCl content (normal, 0.4%; low, 0.04%; or high, 4.0%), and bilateral renal denervation or sham denervation. After 7 days of dietary treatment, all rats were sacrificed and plasma renin activity (PRA) measured. The nNOS and renin messenger RNA (mRNA) levels in the renal cortex were determined by semiquantitative polymerase chain reaction. Results: PRA was higher in animals with low sodium diet compared with those with standard diet, while it was lower in animals with high sodium diet. Renal denervation decreased PRA in normal and low sodium groups, while it did not alter the PRA values in the high sodium group. The nNOS gene expression significantly increase...
Articolo in rivista - Articolo scientifico
Neuronal nitric oxide synthase; Rats; Renal nerves; Renin; Sodium balance;
Renin; Models, Animal; Animals; Sodium, Dietary; Blood Pressure; Denervation; Italy; Stimulation, Chemical; RNA, Messenger; Rats; Rats, Sprague-Dawley; Nitric Oxide Synthase; Neurons; Kidney; Natriuresis; Male
English
ott-2002
20
10
2039
2045
none
Pieruzzi, F., Munforti, C., Di Blasio, A., Busca, G., Dadone, V., Zanchetti, A., et al. (2002). Neuronal nitric oxide synthase and renin stimulation by sodium deprivation are dependent on the renal nerves. JOURNAL OF HYPERTENSION, 20(10), 2039-2045 [10.1097/00004872-200210000-00024].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/39163
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