Analysis of immunoglobulin and T-cell receptor gene rearrangements by real-time quantitative polymerase chain reaction (RQ-PCR) is the gold standard for sensitive and accurate minimal residual disease (MRD) monitoring; it has been extensively standardized and guidelines have been developed within the EuroMRD consortium (www.euromrd.org ). However, new generations of PCR-based methods are standing out as potential alternatives to RQ-PCR, such as digital PCR technology (dPCR), the third-generation implementation of conventional PCR, which has the potential to overcome some of the limitations of RQ-PCR such as allowing the absolute quantification of nucleic acid targets without the need for a calibration curve. During the last years, droplet digital PCR (ddPCR) technology has been compared to RQ-PCR in several hematologic malignancies showing its proficiency for MRD analysis. So far, no established guidelines for ddPCR MRD analysis and data interpretation have been defined and its potential is still under investigation. However, a major standardization effort is underway within the EuroMRD consortium (www.euromrd.org ) for future application of ddPCR in standard clinical practice.

Starza, I., Eckert, C., Drandi, D., Cazzaniga, G. (2022). Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR. In A.W. Langerak (a cura di), Immunogenetics (pp. 79-89). Humana Press Inc. [10.1007/978-1-0716-2115-8_5].

Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR

Cazzaniga, G
2022

Abstract

Analysis of immunoglobulin and T-cell receptor gene rearrangements by real-time quantitative polymerase chain reaction (RQ-PCR) is the gold standard for sensitive and accurate minimal residual disease (MRD) monitoring; it has been extensively standardized and guidelines have been developed within the EuroMRD consortium (www.euromrd.org ). However, new generations of PCR-based methods are standing out as potential alternatives to RQ-PCR, such as digital PCR technology (dPCR), the third-generation implementation of conventional PCR, which has the potential to overcome some of the limitations of RQ-PCR such as allowing the absolute quantification of nucleic acid targets without the need for a calibration curve. During the last years, droplet digital PCR (ddPCR) technology has been compared to RQ-PCR in several hematologic malignancies showing its proficiency for MRD analysis. So far, no established guidelines for ddPCR MRD analysis and data interpretation have been defined and its potential is still under investigation. However, a major standardization effort is underway within the EuroMRD consortium (www.euromrd.org ) for future application of ddPCR in standard clinical practice.
Si
Scientifica
Capitolo o saggio
ddPCR; Immunoglobulin; Minimal residual disease; Rearrangement; RQ-PCR; T-cell receptor;
English
Immunogenetics
978-1-0716-2114-1
eBook ISBN 978-1-0716-2115-8
Starza, I., Eckert, C., Drandi, D., Cazzaniga, G. (2022). Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR. In A.W. Langerak (a cura di), Immunogenetics (pp. 79-89). Humana Press Inc. [10.1007/978-1-0716-2115-8_5].
Starza, I; Eckert, C; Drandi, D; Cazzaniga, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/389768
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