A variety of in vitro techniques are available to estimate the level of antibodies present in human serum samples. Such tests are highly specific and are used to determine prior exposure to a pathogen or to estimate the magnitude, breadth and durability of individual and population level vaccine immunity. Multiplex (or multi-analyte) platforms are increasingly being used to evaluate immune responses against multiple antigens at the same time, usually at reduced per sample cost and a more efficient use of available samples. Consequently, multiplex serology is an essential component of a wide range of public health programmes. Human papillomavirus (HPV) serology is limited to a small number of academic, public health and vaccine manufacturer laboratories globally. Such platforms include indirect binding to the major (L1) capsid protein virus-like particles (VLP), monoclonal antibody competition against L1 VLP and indirect binding to L1 and L2 (minor capsid protein) VLP on multiplex (Luminex®, Meso Scale Discovery®) and standard (ELISA) platforms. The methodology described here utilizes a common multi-analyte platform and L1L2-based VLP expressed in house, which allows the simultaneous detection and quantification of antibody responses against nine vaccine-relevant HPV genotypes.

Panwar, K., Godi, A., Cocuzza, C., Andrews, N., Southern, J., Turner, P., et al. (2022). Multiplex Human Papillomavirus L1L2 virus-like particle antibody binding assay. METHODSX (AMSTERDAM), 9, 1-11 [10.1016/j.mex.2022.101776].

Multiplex Human Papillomavirus L1L2 virus-like particle antibody binding assay

Cocuzza C. E.;
2022

Abstract

A variety of in vitro techniques are available to estimate the level of antibodies present in human serum samples. Such tests are highly specific and are used to determine prior exposure to a pathogen or to estimate the magnitude, breadth and durability of individual and population level vaccine immunity. Multiplex (or multi-analyte) platforms are increasingly being used to evaluate immune responses against multiple antigens at the same time, usually at reduced per sample cost and a more efficient use of available samples. Consequently, multiplex serology is an essential component of a wide range of public health programmes. Human papillomavirus (HPV) serology is limited to a small number of academic, public health and vaccine manufacturer laboratories globally. Such platforms include indirect binding to the major (L1) capsid protein virus-like particles (VLP), monoclonal antibody competition against L1 VLP and indirect binding to L1 and L2 (minor capsid protein) VLP on multiplex (Luminex®, Meso Scale Discovery®) and standard (ELISA) platforms. The methodology described here utilizes a common multi-analyte platform and L1L2-based VLP expressed in house, which allows the simultaneous detection and quantification of antibody responses against nine vaccine-relevant HPV genotypes.
Articolo in rivista - Articolo scientifico
Scientifica
Antibody; Binding; Human papillomavirus; Multiplex Human Papillomavirus L1L2 virus-like particle antibody binding assay; Vaccine;
English
This is an open access article under the CC BY-NC-ND license
Panwar, K., Godi, A., Cocuzza, C., Andrews, N., Southern, J., Turner, P., et al. (2022). Multiplex Human Papillomavirus L1L2 virus-like particle antibody binding assay. METHODSX (AMSTERDAM), 9, 1-11 [10.1016/j.mex.2022.101776].
Panwar, K; Godi, A; Cocuzza, C; Andrews, N; Southern, J; Turner, P; Miller, E; Beddows, S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/387764
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