Live-cell microscopy has highlighted that transcription factors bind transiently to chromatin but it is not clear if the duration of these binding interactions can be modulated in response to an activation stimulus, and if such modulation can be controlled by post-translational modifications of the transcription factor. We address this question for the tumor suppressor p53 by combining live-cell single-molecule microscopy and single cell in situ measurements of transcription and we show that p53-binding kinetics are modulated following genotoxic stress. The modulation of p53 residence times on chromatin requires C-terminal acetylation - a classical mark for transcriptionally active p53 - and correlates with the induction of transcription of target genes such as CDKN1a. We propose a model in which the modification state of the transcription factor determines the coupling between transcription factor abundance and transcriptional activity by tuning the transcription factor residence time on target sites.

Loffreda, A., Jacchetti, E., Antunes, S., Rainone, P., Daniele, T., Morisaki, T., et al. (2017). Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity. NATURE COMMUNICATIONS, 8(1) [10.1038/s41467-017-00398-7].

Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity

Loffreda A.;Rainone P.;
2017

Abstract

Live-cell microscopy has highlighted that transcription factors bind transiently to chromatin but it is not clear if the duration of these binding interactions can be modulated in response to an activation stimulus, and if such modulation can be controlled by post-translational modifications of the transcription factor. We address this question for the tumor suppressor p53 by combining live-cell single-molecule microscopy and single cell in situ measurements of transcription and we show that p53-binding kinetics are modulated following genotoxic stress. The modulation of p53 residence times on chromatin requires C-terminal acetylation - a classical mark for transcriptionally active p53 - and correlates with the induction of transcription of target genes such as CDKN1a. We propose a model in which the modification state of the transcription factor determines the coupling between transcription factor abundance and transcriptional activity by tuning the transcription factor residence time on target sites.
Articolo in rivista - Articolo scientifico
Acetylation; Cell Line, Tumor; Cell Tracking; Chromatin; Cyclin-Dependent Kinase Inhibitor p21; Humans; MCF-7 Cells; Microscopy, Confocal; Promoter Regions, Genetic; Protein Binding; Time Factors; Transcription Factors; Tumor Suppressor Protein p53; Transcription, Genetic;
English
Loffreda, A., Jacchetti, E., Antunes, S., Rainone, P., Daniele, T., Morisaki, T., et al. (2017). Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity. NATURE COMMUNICATIONS, 8(1) [10.1038/s41467-017-00398-7].
Loffreda, A; Jacchetti, E; Antunes, S; Rainone, P; Daniele, T; Morisaki, T; Bianchi, M; Tacchetti, C; Mazza, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/385697
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