Mesenchymal stem cells (MSCs) have been found to be useful in the management of different models of neurological diseases. In the present study, we tested the possible protective effect of MSCs on sensory dorsal root ganglia (DRG) explants exposed to the toxic effect of CDDP, a widely used anticancer drug. DRG explants cultured on a collagen layer and exposed to NGF for 2 h (differentiating neurons) or for 5 days (fully differentiated neurons) were treated with CDDP and subsequently co-cultured with MSCs. MSCs were able to support the survival of both differentiating and fully differentiated DRG neurons up to 2 months after the drug treatment, reducing the CDDP-induced death of DRG neurons. MSCs were, however, unable to restore the correct length of DRG neurites compromised by CDDP treatment. The positive effect on neuronal survival was exerted through the contact between DRG and MSCs, and not mediated by neurotrophic factors released by the MSCs. Our observations could represent a starting point for designing a neuroprotective strategy to limit CDDP induced neuropathy without interfering with its anticancer properties.

Scuteri, A., Donzelli, E., Ravasi, M., Tredici, G. (2008). Adult mesenchymal stem cells support cisplatin-treated dorsal root ganglion survival. NEUROSCIENCE LETTERS, 445(1), 68-72 [10.1016/j.neulet.2008.08.056].

Adult mesenchymal stem cells support cisplatin-treated dorsal root ganglion survival

SCUTERI, ARIANNA;DONZELLI, ELISABETTA;RAVASI, MADDALENA;TREDICI, GIOVANNI
2008

Abstract

Mesenchymal stem cells (MSCs) have been found to be useful in the management of different models of neurological diseases. In the present study, we tested the possible protective effect of MSCs on sensory dorsal root ganglia (DRG) explants exposed to the toxic effect of CDDP, a widely used anticancer drug. DRG explants cultured on a collagen layer and exposed to NGF for 2 h (differentiating neurons) or for 5 days (fully differentiated neurons) were treated with CDDP and subsequently co-cultured with MSCs. MSCs were able to support the survival of both differentiating and fully differentiated DRG neurons up to 2 months after the drug treatment, reducing the CDDP-induced death of DRG neurons. MSCs were, however, unable to restore the correct length of DRG neurites compromised by CDDP treatment. The positive effect on neuronal survival was exerted through the contact between DRG and MSCs, and not mediated by neurotrophic factors released by the MSCs. Our observations could represent a starting point for designing a neuroprotective strategy to limit CDDP induced neuropathy without interfering with its anticancer properties.
Articolo in rivista - Articolo scientifico
Cisplatin; DRG; DRG survival; MSC; Neuroprotective therapy; Peripheral neuropathy;
English
2008
445
1
68
72
none
Scuteri, A., Donzelli, E., Ravasi, M., Tredici, G. (2008). Adult mesenchymal stem cells support cisplatin-treated dorsal root ganglion survival. NEUROSCIENCE LETTERS, 445(1), 68-72 [10.1016/j.neulet.2008.08.056].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/38232
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