In this study we evaluated the effect of recombinant human nerve growth factor (rhNGF) on cisplatin (CDDP)-induced sensory neuronopathy in an experimental paradigm in the rat. Young adult female Wistar rats were treated with CDDP (2 mg/kg ip twice weekly for nine times) alone or in combination with rhNGF (1 mg/kg sc on alternate days). The effect of CDDP +/- NGF treatment was evaluated with behavioral (tail-flick test) and neurophysiological (nerve conduction velocity in the tail) methods immediately after treatment and after a follow-up period of 6 weeks. Pathological and morphometrical examinations of the dorsal root ganglia (DRG) and sciatic and saphenous nerves were also performed. rhNGF treatment induced a significant reduction in the CDDP-induced decrease in nerve conduction velocity (P < 0.05), and this was associated with a significant protection against the decrease in somatic (P < 0.05), nuclear (P < 0.05), and nucleolar size (P < 0.01) caused by CDDP treatment. However, for each of the parameters examined the neuroprotection obtained with rhNGF treatment was not complete. At the follow-up examination no differences between the three groups were observed in tail-flick test and nerve conduction velocity. We conclude that rhNGF, administered according to the schedule used in this experiment, exerts a biologically significant neuroprotective effect against CDDP peripheral neurotoxicity

Tredici, G., Braga, M., Nicolini, G., Miloso, M., Marmiroli, P., Schenone, A., et al. (1999). Effect of recombinant human nerve growth factor on cisplatin neurotoxicity in rats. EXPERIMENTAL NEUROLOGY, 159(2), 551-558 [10.1006/exnr.1999.7174].

Effect of recombinant human nerve growth factor on cisplatin neurotoxicity in rats

TREDICI, GIOVANNI;NICOLINI, GABRIELLA;MILOSO, MARIAROSARIA;MARMIROLI, PAOLA LORENA;CAVALETTI, GUIDO ANGELO
1999

Abstract

In this study we evaluated the effect of recombinant human nerve growth factor (rhNGF) on cisplatin (CDDP)-induced sensory neuronopathy in an experimental paradigm in the rat. Young adult female Wistar rats were treated with CDDP (2 mg/kg ip twice weekly for nine times) alone or in combination with rhNGF (1 mg/kg sc on alternate days). The effect of CDDP +/- NGF treatment was evaluated with behavioral (tail-flick test) and neurophysiological (nerve conduction velocity in the tail) methods immediately after treatment and after a follow-up period of 6 weeks. Pathological and morphometrical examinations of the dorsal root ganglia (DRG) and sciatic and saphenous nerves were also performed. rhNGF treatment induced a significant reduction in the CDDP-induced decrease in nerve conduction velocity (P < 0.05), and this was associated with a significant protection against the decrease in somatic (P < 0.05), nuclear (P < 0.05), and nucleolar size (P < 0.01) caused by CDDP treatment. However, for each of the parameters examined the neuroprotection obtained with rhNGF treatment was not complete. At the follow-up examination no differences between the three groups were observed in tail-flick test and nerve conduction velocity. We conclude that rhNGF, administered according to the schedule used in this experiment, exerts a biologically significant neuroprotective effect against CDDP peripheral neurotoxicity
Articolo in rivista - Articolo scientifico
cisplatin; nerve growth factor; peripheral neuropathy; neuroprotection; neurophysiology; pathology
English
551
558
8
Tredici, G., Braga, M., Nicolini, G., Miloso, M., Marmiroli, P., Schenone, A., et al. (1999). Effect of recombinant human nerve growth factor on cisplatin neurotoxicity in rats. EXPERIMENTAL NEUROLOGY, 159(2), 551-558 [10.1006/exnr.1999.7174].
Tredici, G; Braga, M; Nicolini, G; Miloso, M; Marmiroli, P; Schenone, A; Nobbio, L; Frattola, L; Cavaletti, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/38142
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