Novel and complementary experimental models are required for investigating the molecular mechanisms underlying the resistance to the available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third of patients and need to be deeply investigated. Here, we have established a patient-specific disease model for TRD by reprogramming peripheral blood mononuclear cells (PBMCs) from two TRD patients into induced pluripotent stem cells (iPSCs), using non-integrating Sendai virus. These lines show the typical morphology of pluripotent cells, express pluripotency markers and displayed in vitro differentiation potential toward cells of the three embryonic germ layers.

Bono, F., Mutti, V., Piovani, G., Minelli, A., Mingardi, J., Guglielmi, A., et al. (2020). Generation of two human induced pluripotent stem cell lines, UNIBSi012-A and UNIBSi013-A, from two patients with treatment-resistant depression. STEM CELL RESEARCH, 49(December 2020) [10.1016/j.scr.2020.102104].

Generation of two human induced pluripotent stem cell lines, UNIBSi012-A and UNIBSi013-A, from two patients with treatment-resistant depression

Mingardi J.;
2020

Abstract

Novel and complementary experimental models are required for investigating the molecular mechanisms underlying the resistance to the available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third of patients and need to be deeply investigated. Here, we have established a patient-specific disease model for TRD by reprogramming peripheral blood mononuclear cells (PBMCs) from two TRD patients into induced pluripotent stem cells (iPSCs), using non-integrating Sendai virus. These lines show the typical morphology of pluripotent cells, express pluripotency markers and displayed in vitro differentiation potential toward cells of the three embryonic germ layers.
Articolo in rivista - Articolo scientifico
adult; Article; cell differentiation; cell reprogramming technique; cell structure; embryo; female; genomic instability; germ layer; human; human cell; in vitro study; induced pluripotent stem cell; karyotyping; middle aged; nonhuman; peripheral blood mononuclear cell; priority journal; Sendai virus; stem cell self-renewal; treatment resistant depression; UNIBSi012 A cell line; UNIBSi013 A cell line
English
Bono, F., Mutti, V., Piovani, G., Minelli, A., Mingardi, J., Guglielmi, A., et al. (2020). Generation of two human induced pluripotent stem cell lines, UNIBSi012-A and UNIBSi013-A, from two patients with treatment-resistant depression. STEM CELL RESEARCH, 49(December 2020) [10.1016/j.scr.2020.102104].
Bono, F; Mutti, V; Piovani, G; Minelli, A; Mingardi, J; Guglielmi, A; Fiorentini, C; Barbon, A; Missale, C; Gennarelli, M
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S1873506120304050-main.pdf

accesso aperto

Dimensione 2.45 MB
Formato Adobe PDF
2.45 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/376695
Citazioni
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
Social impact