The effect of galanin (Gal), a 29-amino acid peptide widely distributed in mammalian CNS, was investigated in cultured pituitary cells of rats of different ages. Gal (0.1-10/microM) stimulated GH release in a concentration-dependent manner in 5-and 10-day-old rat pituitaries (EC50: 0.87 and 1.73/microM, respectively) but was ineffective (0.01-1/microM) or even inhibitory (10/microM) in 40-day-old male rat pituitaries. Gal (0.1-10/microM) was ineffective to alter stimulation of GH release induced by GHRH (10 nM) in 5-day-and 40-day-old rat pituitary cells, but Gal (1/microM) slightly inhibited (24%) it in 10-day-old rat pituitaries. Gal (1 and 10/microM) also inhibited GH secretion (45 and 58%, respectively) from 40-day-old pituitary cells when a lower GHRH dose (0.1 nM) was used for stimulation. The results of this study indicate that Gal has the ability to either stimulate or inhibit GH release from dispersed pituitary cells and that its effects are closely related to the age of the rats

Torsello, A., Sellan, R., Cella, S., Locatelli, V., & Müller, E. (1990). Age-dependent modulation by galanin of growth hormone release from rat pituitary cells in culture. LIFE SCIENCES, 47(20), 1861-1866 [10.1016/0024-3205(90)90289-4].

Age-dependent modulation by galanin of growth hormone release from rat pituitary cells in culture

TORSELLO, ANTONIO BIAGIO;LOCATELLI, VITTORIO;
1990

Abstract

The effect of galanin (Gal), a 29-amino acid peptide widely distributed in mammalian CNS, was investigated in cultured pituitary cells of rats of different ages. Gal (0.1-10/microM) stimulated GH release in a concentration-dependent manner in 5-and 10-day-old rat pituitaries (EC50: 0.87 and 1.73/microM, respectively) but was ineffective (0.01-1/microM) or even inhibitory (10/microM) in 40-day-old male rat pituitaries. Gal (0.1-10/microM) was ineffective to alter stimulation of GH release induced by GHRH (10 nM) in 5-day-and 40-day-old rat pituitary cells, but Gal (1/microM) slightly inhibited (24%) it in 10-day-old rat pituitaries. Gal (1 and 10/microM) also inhibited GH secretion (45 and 58%, respectively) from 40-day-old pituitary cells when a lower GHRH dose (0.1 nM) was used for stimulation. The results of this study indicate that Gal has the ability to either stimulate or inhibit GH release from dispersed pituitary cells and that its effects are closely related to the age of the rats
Articolo in rivista - Articolo scientifico
Scientifica
Rats; Galanin; Animals; Pituitary Gland; Dose-Response Relationship, Drug; Cells, Cultured; Aging; Peptides; Growth Hormone-Releasing Hormone; Male; Female; Growth Hormone
English
Torsello, A., Sellan, R., Cella, S., Locatelli, V., & Müller, E. (1990). Age-dependent modulation by galanin of growth hormone release from rat pituitary cells in culture. LIFE SCIENCES, 47(20), 1861-1866 [10.1016/0024-3205(90)90289-4].
Torsello, A; Sellan, R; Cella, S; Locatelli, V; Müller, E
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/37143
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