An emerging area of biosensors is based on the use of structures provided by recent advances ofNanotechnology such as nanowires, nanotubes and nanopores. Among them, the integration of natural nanopores such as ion channels with electronics is a promising approach to develop rapid, sensitive and reliable biosensors able to detect low concentration of target molecules or DNA sequencing. This paper presents a compact and low-cost system able to readout, process and record current in the pA range, provided by biological or synthetic nanopores. The approach is based on the idea that by processing the outputs of a large amount of single-molecule nanosensors would result in a significant increase of resolution and signal-to-noise ratio. The approach consists of an electronic interface able to detect current-based array of nanosensors, where the management of very large amount of data is critical for the readout process. As working example, we acquired the single molecule signals derived from non-covalent bindings between single a-hemolysin pores, embedded into an artificial lipid bilayer, and β-cyclodextrin molecules. The system embeds the electronic readout with the microfluidic where is placed the nanosensor array. The electronic interface is a 0.5mm2 current amplifier based on an array of Lσ△ converters. Then the high rate data streams are processed and downsampled by a DSP that communicates with a PC via a USB interface for data processing and storage.

Crescentini, M., Rossi, M., Bennati, M., Thei, F., Baschirotto, A., Tartagni, M. (2009). A nanosensor interface based on delta-sigma arrays. In 3rd International Workshop on Advances in sensors and Interfaces. IWASI 2009 (pp.163-167). IEEE [10.1109/IWASI.2009.5184788].

A nanosensor interface based on delta-sigma arrays

BASCHIROTTO, ANDREA;
2009

Abstract

An emerging area of biosensors is based on the use of structures provided by recent advances ofNanotechnology such as nanowires, nanotubes and nanopores. Among them, the integration of natural nanopores such as ion channels with electronics is a promising approach to develop rapid, sensitive and reliable biosensors able to detect low concentration of target molecules or DNA sequencing. This paper presents a compact and low-cost system able to readout, process and record current in the pA range, provided by biological or synthetic nanopores. The approach is based on the idea that by processing the outputs of a large amount of single-molecule nanosensors would result in a significant increase of resolution and signal-to-noise ratio. The approach consists of an electronic interface able to detect current-based array of nanosensors, where the management of very large amount of data is critical for the readout process. As working example, we acquired the single molecule signals derived from non-covalent bindings between single a-hemolysin pores, embedded into an artificial lipid bilayer, and β-cyclodextrin molecules. The system embeds the electronic readout with the microfluidic where is placed the nanosensor array. The electronic interface is a 0.5mm2 current amplifier based on an array of Lσ△ converters. Then the high rate data streams are processed and downsampled by a DSP that communicates with a PC via a USB interface for data processing and storage.
paper
DNA sequencing; USB interface; artificial lipid bilayer; beta-cyclodextrin; biosensors; data processing; data storage; delta sigma arrays; electronic readout; ion channels; nanopores; nanosensor interface; nanotubes; nanowires; DNA; biosensors; digital signal processing chips; nanobiotechnology; readout electronics
English
ISSCC
3rd International Workshop on Advances in sensors and Interfaces. IWASI 2009
978-1-4244-4708-4
2009
163
167
none
Crescentini, M., Rossi, M., Bennati, M., Thei, F., Baschirotto, A., Tartagni, M. (2009). A nanosensor interface based on delta-sigma arrays. In 3rd International Workshop on Advances in sensors and Interfaces. IWASI 2009 (pp.163-167). IEEE [10.1109/IWASI.2009.5184788].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/36859
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