The gut can be a source of sepsis after thermal injury. In the present study the relationship between the extent of burn injury and magnitude of bacterial translocation was investigated. Mice underwent 0%, 10%, 20%, 30%, or 50% total body surface area full-thickness burn and simultaneous gavage with 1 x 10(10) 14C-labeled Escherichia coli. mesenteric lymph nodes, liver, spleen, peritoneal fluid, and burn wound were excised 4 hours after burn injury. Residual radioactivity and bacterial colony counts were measured, and percentages of viable organisms were calculated. Results showed that the rate of translocation of 14C E. coli increased proportionally with the burn size, reaching a maximum at 30%. The cutaneous eschar collected a remarkable amount of labeled bacteria, suggesting enteric microflora as a possible source of contamination of the burn wound via endogenous routes. The percentage of viable organisms in the tissues demonstrated that the ability of mesenteric lymph nodes, liver, and eschar to clear translocated bacteria was directly affected by the severity of the burn injury.

Gianotti, L., Alexander, J., Pyles, T., James, L., Babcock, G. (1993). Relationship between extent of burn injury and magnitude of microbial translocation from the intestine. JOURNAL OF BURN CARE & REHABILITATION, 14(3), 336-342.

Relationship between extent of burn injury and magnitude of microbial translocation from the intestine

GIANOTTI, LUCA VITTORIO;
1993-05

Abstract

The gut can be a source of sepsis after thermal injury. In the present study the relationship between the extent of burn injury and magnitude of bacterial translocation was investigated. Mice underwent 0%, 10%, 20%, 30%, or 50% total body surface area full-thickness burn and simultaneous gavage with 1 x 10(10) 14C-labeled Escherichia coli. mesenteric lymph nodes, liver, spleen, peritoneal fluid, and burn wound were excised 4 hours after burn injury. Residual radioactivity and bacterial colony counts were measured, and percentages of viable organisms were calculated. Results showed that the rate of translocation of 14C E. coli increased proportionally with the burn size, reaching a maximum at 30%. The cutaneous eschar collected a remarkable amount of labeled bacteria, suggesting enteric microflora as a possible source of contamination of the burn wound via endogenous routes. The percentage of viable organisms in the tissues demonstrated that the ability of mesenteric lymph nodes, liver, and eschar to clear translocated bacteria was directly affected by the severity of the burn injury.
Articolo in rivista - Articolo scientifico
Scientifica
Burns; Animals; Skin; Spleen; Mice; Mice, Inbred BALB C; Intestines; Ascitic Fluid; Escherichia coli; Liver; Colony Count, Microbial; Lymph Nodes; Female
English
Gianotti, L., Alexander, J., Pyles, T., James, L., Babcock, G. (1993). Relationship between extent of burn injury and magnitude of microbial translocation from the intestine. JOURNAL OF BURN CARE & REHABILITATION, 14(3), 336-342.
Gianotti, L; Alexander, J; Pyles, T; James, L; Babcock, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/36681
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