The aim of this study was to evaluate the ability of basic fibroblast growth factor (bFGF) and sucralfate to prevent bacterial translocation after burn injury. Four groups of Balb/c mice (n = 10/group) were treated by gavage with bFGF (10 micrograms/kg/d), sucralfate (15 mg/kg/d), bFGF plus sucralfate, or saline for 4 days prior to receiving a gavage with 1 x 10(10) 14C-radiolabeled Escherichia coli and a 20% full-thickness burn. Four hours after burn, the mesenteric lymph nodes, liver, spleen, and blood were harvested aseptically. For each tissue, the number of viable bacteria and radionuclide counts of the translocated 14C-labeled E. coli were measured, and the percentage of translocated organisms that remained alive was calculated. The results indicated that treatment with either bFGF or sucralfate alone had a partial effect on translocation, whereas the combined treatment with bFGF plus sucralfate significantly decreased the magnitude of translocation in all tested tissues (p < 0.05, ANOVA), which was associated with complete preservation of gut mucosal integrity. None of the treatments affected the ability of the host to kill translocated bacteria when the results were compared with those of the controls. The additive effect of the combined therapy may be due to the high affinity of sucralfate for bFGF, decreasing the degradation of bFGF by gastric acid.

Gianotti, L., Alexander, J., Fukushima, R., & Pyles, T. (1993). Reduction of bacterial translocation with oral fibroblast growth factor and sucralfate. THE AMERICAN JOURNAL OF SURGERY, 165(1), 195-201.

Reduction of bacterial translocation with oral fibroblast growth factor and sucralfate

GIANOTTI, LUCA VITTORIO;
1993-01

Abstract

The aim of this study was to evaluate the ability of basic fibroblast growth factor (bFGF) and sucralfate to prevent bacterial translocation after burn injury. Four groups of Balb/c mice (n = 10/group) were treated by gavage with bFGF (10 micrograms/kg/d), sucralfate (15 mg/kg/d), bFGF plus sucralfate, or saline for 4 days prior to receiving a gavage with 1 x 10(10) 14C-radiolabeled Escherichia coli and a 20% full-thickness burn. Four hours after burn, the mesenteric lymph nodes, liver, spleen, and blood were harvested aseptically. For each tissue, the number of viable bacteria and radionuclide counts of the translocated 14C-labeled E. coli were measured, and the percentage of translocated organisms that remained alive was calculated. The results indicated that treatment with either bFGF or sucralfate alone had a partial effect on translocation, whereas the combined treatment with bFGF plus sucralfate significantly decreased the magnitude of translocation in all tested tissues (p < 0.05, ANOVA), which was associated with complete preservation of gut mucosal integrity. None of the treatments affected the ability of the host to kill translocated bacteria when the results were compared with those of the controls. The additive effect of the combined therapy may be due to the high affinity of sucralfate for bFGF, decreasing the degradation of bFGF by gastric acid.
Articolo in rivista - Articolo scientifico
Scientifica
Burns; Cell Movement; Animals; Sucralfate; Carbon Radioisotopes; Digestive System; Spleen; Mice; Mice, Inbred BALB C; Fibroblast Growth Factor 2; Mesentery; Escherichia coli Infections; Escherichia coli; Liver; Lymph Nodes; Female
English
Gianotti, L., Alexander, J., Fukushima, R., & Pyles, T. (1993). Reduction of bacterial translocation with oral fibroblast growth factor and sucralfate. THE AMERICAN JOURNAL OF SURGERY, 165(1), 195-201.
Gianotti, L; Alexander, J; Fukushima, R; Pyles, T
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/36680
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