The role of the immune system in microbial translocation must be clarified. In these studies, the effect of blood transfusion-related immunosuppression on translocation was investigated in a burn animal model previously known to increase the gut's permeability to 14C-radiolabeled Escherichia coli. In a first experiment, Balb/c mice underwent transfusion (T) with 0.2 mL per mouse of allogeneic C3H/HeJ mouse blood 5 days prior to undergoing 30-percent burn injury (B) and simultaneous gavage (G) with 10(9) E. coli bacteria labeled with 14C. An additional six groups of Balb/c mice underwent different combinations of T, B, and G procedures (TG, BG, TB, T, B, G). Survival rate was recorded for all groups on Day 10. This experiment suggested that B and T, to a lesser extent, were the factors affecting survival, although the combination of T, B, and G clearly showed a synergistic effect on mortality. In a second experiment, 18 Balb/c mice belonging to TBG, BG, TG, and G groups were sacrificed 1, 4, and 24 hours after burn or gavage. The residual radioactivity and the percentage of viable bacteria were computed for mesenteric lymph nodes, spleen, liver, lungs, blood, and peritoneal fluid. Statistical analysis of the radionuclide counts recognized B as the only variable able to enhance the magnitude of 14C E. coli translocation. The percentage of viable bacteria showed that T and, more moderately, B were the factors leading to the failure of bacterial clearance in the tissues.
Gianotti, L.V., Pyles, T., Alexander, J., Babcock, G., & Carey, M. (1992). Impact of blood transfusion and burn injury on microbial translocation and bacterial survival. TRANSFUSION, 32(4), 312-317.
|Citazione:||Gianotti, L.V., Pyles, T., Alexander, J., Babcock, G., & Carey, M. (1992). Impact of blood transfusion and burn injury on microbial translocation and bacterial survival. TRANSFUSION, 32(4), 312-317.|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Presenza di un coautore afferente ad Istituzioni straniere:||Si|
|Titolo:||Impact of blood transfusion and burn injury on microbial translocation and bacterial survival|
|Autori:||Gianotti, LV; Pyles, T; Alexander, J; Babcock, G; Carey, M|
|Data di pubblicazione:||1992|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1046/j.1537-2995.1992.32492263443.x|
|Appare nelle tipologie:||01 - Articolo su rivista|