The process of microbial translocation was studied using Candida albicans, Escherichia coli, or endotoxin instilled into Thiry-Vella loops of thermally injured guinea pigs and rats. Translocation of C. albicans occurred by direct penetration of enterocytes by a unique process different from classical phagocytosis. Translocation between enterocytes was not observed. Internalization was associated with a disturbance of the plasma membrane and brush border, but most internalized organisms were not surrounded by a plasma membrane. Passage of the candida into the lamina propria appeared to be associated with disruption of the basal membrane with extrusion of cytoplasm of the cell and candida. Organisms in the lamina propria were commonly phagocytized by macrophages but also were found free in lymphatics and blood vessels. Translocation of E. coli and endotoxin also occurred directly through enterocytes rather than between them, but translocated endotoxin diffused through the lamina propria and muscular wall of the bowel wall by passing between rather than through the myocytes. These descriptive phenomena provide new insight into the role of the enterocyte and intestinal immune cells in the translocation process.

Alexander, J., Boyce, S., Babcock, G., Gianotti, L., Peck, M., Dunn, D., et al. (1990). The process of microbial translocation. ANNALS OF SURGERY, 212(4), 496-512.

The process of microbial translocation

GIANOTTI, LUCA VITTORIO;
1990-10

Abstract

The process of microbial translocation was studied using Candida albicans, Escherichia coli, or endotoxin instilled into Thiry-Vella loops of thermally injured guinea pigs and rats. Translocation of C. albicans occurred by direct penetration of enterocytes by a unique process different from classical phagocytosis. Translocation between enterocytes was not observed. Internalization was associated with a disturbance of the plasma membrane and brush border, but most internalized organisms were not surrounded by a plasma membrane. Passage of the candida into the lamina propria appeared to be associated with disruption of the basal membrane with extrusion of cytoplasm of the cell and candida. Organisms in the lamina propria were commonly phagocytized by macrophages but also were found free in lymphatics and blood vessels. Translocation of E. coli and endotoxin also occurred directly through enterocytes rather than between them, but translocated endotoxin diffused through the lamina propria and muscular wall of the bowel wall by passing between rather than through the myocytes. These descriptive phenomena provide new insight into the role of the enterocyte and intestinal immune cells in the translocation process.
Articolo in rivista - Articolo scientifico
Scientifica
Burns; Cell Movement; Endotoxins; Animals; Rats, Inbred Lew; Guinea Pigs; Biological Transport; Candida albicans; Intestinal Mucosa; Infection; Rats; Microscopy, Fluorescence; Escherichia coli; Basement Membrane; Microvilli; Female; Male; Microscopy, Electron, Scanning
English
Alexander, J., Boyce, S., Babcock, G., Gianotti, L., Peck, M., Dunn, D., et al. (1990). The process of microbial translocation. ANNALS OF SURGERY, 212(4), 496-512.
Alexander, J; Boyce, S; Babcock, G; Gianotti, L; Peck, M; Dunn, D; Pyles, T; Childress, C; Ash, S
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/36672
Citazioni
  • Scopus 414
  • ???jsp.display-item.citation.isi??? 377
Social impact