IRIS – Institutional Research Information System
IRIS è il sistema di gestione della ricerca dell’Università degli Studi di Milano-Bicocca.Il repository BOA è il modulo del sistema dedicato alla raccolta e alla disseminazione della produzione scientifica di Ateneo. Le pubblicazioni sono ricercabili per parola chiave attraverso il box nella barra orizzontale in alto oppure, mediante il pulsante Sfoglia, per "Afferenza", "Autore", "Titolo", "Tipologia" o "Settore scientifico-disciplinare".
IRIS contiene inoltre alcuni moduli dedicati alla registrazione dei progetti e dei contratti, e alla reportistica avanzata per le attività di valutazione della ricerca
EnglishThe whole cell biological conversion of naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene by the E. coli JM109(pPS1778) recombinant strain carrying the naphthalene dioxygenase and regulatory genes cloned from Pseudomonas fluorescens N3 in micellar systems has been investigated using biochemical and chemico-physical techniques. Reverse and direct micellar systems have been tested. Non-ionic surfactants (Tween and Triton X series) were found not to inhibit either the growth of the bacteria and the expression of the hydroxylating dioxygenase enzyme in such systems and were utilized in order to speed up the naphthalene conversion by increasing its solubility and also its bioavailability. The phase behavior of the direct micellar system was characterized through light scattering and other chemico-physical techniques. Further addition of isopropyl-palmitate 1-2% v/v to the micellar systems resulted in an increase of the apparent substrate concentration in solution and particularly its bioavailability thus allowing faster catalytic conversions resulting in an increase in productivity for the process. Since the cis-dihydrodiols are acquiring considerable potential as chiral pool synthons in asymmetric synthesis for a variety of industrial processes, possible applications for efficient small and large-scale production of such compounds are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.
Briganti, F., Randazzo, D., Scozzafava, A., Berti, D., Baglioni, P., Di Gennaro, P., et al. (1999). Characterization of the biological conversion of naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene in direct micellar systems. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 7(5-6), 263-272 [10.1016/S1381-1177(99)00046-6].
| Citazione: | Briganti, F., Randazzo, D., Scozzafava, A., Berti, D., Baglioni, P., Di Gennaro, P., et al. (1999). Characterization of the biological conversion of naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene in direct micellar systems. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 7(5-6), 263-272 [10.1016/S1381-1177(99)00046-6]. | |
| Tipo: | Articolo in rivista - Articolo scientifico | |
| Carattere della pubblicazione: | Scientifica | |
| Titolo: | Characterization of the biological conversion of naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene in direct micellar systems | |
| Autori: | Briganti, F; Randazzo, D; Scozzafava, A; Berti, D; Baglioni, P; Di Gennaro, P; Galli, E; Bestetti, G | |
| Autori: | ||
| Data di pubblicazione: | 1999 | |
| Lingua: | English | |
| Rivista: | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S1381-1177(99)00046-6 | |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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