Surgical excision followed by histopathological examination is the gold standard for melanoma screening. However, the color-based inspection of hematoxylin-and-eosin-stained biopsies does not provide a space-resolved quantification of the melanin content in melanocytic lesions. We propose a non-destructive photo-thermal imaging method capable of characterizing the microscopic distribution and absolute concentration of melanin pigments in excised melanoma biopsies. By exploiting the photo-thermal effect primed by melanin absorption of visible laser light we obtain label-free super-resolution far-infrared thermal images of tissue sections where melanin is spatially mapped at sub-diffraction 40-µm resolution. Based on the finite-element simulation of the full 3D heat transfer model, we are able to convert temperature maps into quantitative images of the melanin molar concentration on B16 murine melanoma biopsies, with 4·10−4 M concentration sensitivity. Being readily applicable to human melanoma biopsies in combination with hematoxylin-and-eosin staining, the proposed approach could complement traditional histopathology in the characterization of pigmented lesions ex-vivo.

Bouzin, M., Marini, M., Chirico, G., Granucci, F., Mingozzi, F., Colombo, R., et al. (2022). Melanin concentration maps by label-free super-resolution photo-thermal imaging on melanoma biopsies. BIOMEDICAL OPTICS EXPRESS, 13(3 (1 March 2022)), 1173-1187 [10.1364/BOE.445945].

Melanin concentration maps by label-free super-resolution photo-thermal imaging on melanoma biopsies

Bouzin M.
Co-primo
;
Marini M.
Co-primo
;
Chirico G.
;
Granucci F.;Mingozzi F.;Colombo R.;D'Alfonso L.;Sironi L.;Collini M.
Ultimo
2022

Abstract

Surgical excision followed by histopathological examination is the gold standard for melanoma screening. However, the color-based inspection of hematoxylin-and-eosin-stained biopsies does not provide a space-resolved quantification of the melanin content in melanocytic lesions. We propose a non-destructive photo-thermal imaging method capable of characterizing the microscopic distribution and absolute concentration of melanin pigments in excised melanoma biopsies. By exploiting the photo-thermal effect primed by melanin absorption of visible laser light we obtain label-free super-resolution far-infrared thermal images of tissue sections where melanin is spatially mapped at sub-diffraction 40-µm resolution. Based on the finite-element simulation of the full 3D heat transfer model, we are able to convert temperature maps into quantitative images of the melanin molar concentration on B16 murine melanoma biopsies, with 4·10−4 M concentration sensitivity. Being readily applicable to human melanoma biopsies in combination with hematoxylin-and-eosin staining, the proposed approach could complement traditional histopathology in the characterization of pigmented lesions ex-vivo.
Articolo in rivista - Articolo scientifico
Scientifica
Melanin; label-free; photo-thermal microscopy;
English
Bouzin, M., Marini, M., Chirico, G., Granucci, F., Mingozzi, F., Colombo, R., et al. (2022). Melanin concentration maps by label-free super-resolution photo-thermal imaging on melanoma biopsies. BIOMEDICAL OPTICS EXPRESS, 13(3 (1 March 2022)), 1173-1187 [10.1364/BOE.445945].
Bouzin, M; Marini, M; Chirico, G; Granucci, F; Mingozzi, F; Colombo, R; D'Alfonso, L; Sironi, L; Collini, M
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/359866
Citazioni
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 0
Social impact