Radiolabeled monoclonal antibodies (MAbs) have been reported to allow tumor intraoperative detection by means of a gamma-detecting probe. The technology is called the Radioimmunoguided Surgery (RIGS) system. The main inconveniences of the method are 1) the long interval needed for clearance of unattached MAbs from the patient's body, between the injection of the MAb and surgery, and 2) the low sensitivity of current MAbs used in detecting small tumors. We describe a new method to overcome these inconveniences using biotinylated MAbs and avidin in order to obtain a rapid blood clearance of the radiolabeled MAbs both anticarcinoembryonic antigen and antitumor-associated glycoprotein-72 MAbs.

Stella, M., De Nardi, P., Paganelli, G., Magnani, P., Mangili, F., Sassi, I., et al. (1994). Avidin-biotin system in radioimmunoguided surgery for colorectal cancer. Advantages and limits. DISEASES OF THE COLON & RECTUM, 37(4), 335-343 [10.1007/BF02053593].

Avidin-biotin system in radioimmunoguided surgery for colorectal cancer. Advantages and limits

FAZIO, FERRUCCIO;
1994

Abstract

Radiolabeled monoclonal antibodies (MAbs) have been reported to allow tumor intraoperative detection by means of a gamma-detecting probe. The technology is called the Radioimmunoguided Surgery (RIGS) system. The main inconveniences of the method are 1) the long interval needed for clearance of unattached MAbs from the patient's body, between the injection of the MAb and surgery, and 2) the low sensitivity of current MAbs used in detecting small tumors. We describe a new method to overcome these inconveniences using biotinylated MAbs and avidin in order to obtain a rapid blood clearance of the radiolabeled MAbs both anticarcinoembryonic antigen and antitumor-associated glycoprotein-72 MAbs.
Articolo in rivista - Articolo scientifico
Sensitivity and Specificity; Preoperative Care; Humans; Predictive Value of Tests; False Positive Reactions; Antibodies, Monoclonal; False Negative Reactions; Avidin; Intraoperative Care; Treatment Outcome; Radioimmunodetection; Iodine Radioisotopes; Surgical Procedures, Operative; Neoplasm Recurrence, Local; Injections; Time Factors; Biotin; Colorectal Neoplasms
English
1994
37
4
335
343
none
Stella, M., De Nardi, P., Paganelli, G., Magnani, P., Mangili, F., Sassi, I., et al. (1994). Avidin-biotin system in radioimmunoguided surgery for colorectal cancer. Advantages and limits. DISEASES OF THE COLON & RECTUM, 37(4), 335-343 [10.1007/BF02053593].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/35515
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