SMYD3 (SET and MYND domain containing protein 3) is a methylase over-expressed in cancer cells and involved in oncogenesis. While several studies uncovered key functions for SMYD3 in cancer models, the SMYD3 role in physiological conditions has not been fully elucidated yet. Here, we dissect the role of SMYD3 at early stages of development, employing mouse embryonic stem cells (ESCs) and zebrafish as model systems. We report that SMYD3 depletion promotes the induction of the mesodermal pattern during in vitro differentiation of ESCs and is linked to an upregulation of cardiovascular lineage markers at later stages. In vivo, smyd3 knockdown in zebrafish favors the upregulation of mesendodermal markers during zebrafish gastrulation. Overall, our study reveals that SMYD3 modulates levels of mesendodermal markers, both in development and in embryonic stem cell differentiation.

Fittipaldi, R., Floris, P., Proserpio, V., Cotelli, F., Beltrame, M., Caretti, G. (2021). The lysine methylase smyd3 modulates mesendodermal commitment during development. CELLS, 10(5) [10.3390/cells10051233].

The lysine methylase smyd3 modulates mesendodermal commitment during development

Floris P.
Secondo
;
2021

Abstract

SMYD3 (SET and MYND domain containing protein 3) is a methylase over-expressed in cancer cells and involved in oncogenesis. While several studies uncovered key functions for SMYD3 in cancer models, the SMYD3 role in physiological conditions has not been fully elucidated yet. Here, we dissect the role of SMYD3 at early stages of development, employing mouse embryonic stem cells (ESCs) and zebrafish as model systems. We report that SMYD3 depletion promotes the induction of the mesodermal pattern during in vitro differentiation of ESCs and is linked to an upregulation of cardiovascular lineage markers at later stages. In vivo, smyd3 knockdown in zebrafish favors the upregulation of mesendodermal markers during zebrafish gastrulation. Overall, our study reveals that SMYD3 modulates levels of mesendodermal markers, both in development and in embryonic stem cell differentiation.
Articolo in rivista - Articolo scientifico
Development; Embryonic stem cells; SMYD3; Zebrafish;
English
Fittipaldi, R., Floris, P., Proserpio, V., Cotelli, F., Beltrame, M., Caretti, G. (2021). The lysine methylase smyd3 modulates mesendodermal commitment during development. CELLS, 10(5) [10.3390/cells10051233].
Fittipaldi, R; Floris, P; Proserpio, V; Cotelli, F; Beltrame, M; Caretti, G
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/352922
Citazioni
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
Social impact