Animal studies of infection imaging by a two-step protocol have shown that important improvements in target to nontarget ratios are possible. In this protocol, unlabeled streptavidin is administered and allowed sufficient time to accumulate in the lesion, probably by nonspecific processes, and to clear elsewhere. Thereafter, In-111-biotin is administered. A fraction of the labeled biotin may be retained in the lesion because of biotin's high affinity for streptavidin while most of the activity is cleared through the kidneys, Methods: Radioscintigraphy with unlabeled streptavidin followed with In-111-labeled biotin was performed in 15 patients with chronic osteomyelitis. As controls, each patients received either In-111-labeled biotin without the preadministration of streptavidin or In-111-labeled non-specific IgG, Results: Regions of focal uptake were identified in all patients receiving streptavidin followed by radiolabeled biotin as early as 10 min postadministration of radioactivity, and retention of label was evident through 24 hr. Coincident regions of abnormal accumulation were apparent with In-111-lgG, but only in delayed images, Moreover, with In-111-biotin alone, without the preadministration of streptavidin, focal accumulations were detected in areas similar to that identified with the two-step protocol. Although, these observations were only in the earliest images. Conclusion: The results of this preliminary clinical investigation suggest that a two-step protocol with unlabeled streptavidin and radiolabeled biotin may be an alternative for the detection of infection

Rusckowski, M., Paganelli, G., Hnatowich, D., Magnani, P., Virzi, F., Fogarasi, M., et al. (1996). Imaging osteomyelitis with streptavidin and Indium-111-labeled biotin. THE JOURNAL OF NUCLEAR MEDICINE, 37(10), 1655-1662.

Imaging osteomyelitis with streptavidin and Indium-111-labeled biotin

Fazio, F
1996

Abstract

Animal studies of infection imaging by a two-step protocol have shown that important improvements in target to nontarget ratios are possible. In this protocol, unlabeled streptavidin is administered and allowed sufficient time to accumulate in the lesion, probably by nonspecific processes, and to clear elsewhere. Thereafter, In-111-biotin is administered. A fraction of the labeled biotin may be retained in the lesion because of biotin's high affinity for streptavidin while most of the activity is cleared through the kidneys, Methods: Radioscintigraphy with unlabeled streptavidin followed with In-111-labeled biotin was performed in 15 patients with chronic osteomyelitis. As controls, each patients received either In-111-labeled biotin without the preadministration of streptavidin or In-111-labeled non-specific IgG, Results: Regions of focal uptake were identified in all patients receiving streptavidin followed by radiolabeled biotin as early as 10 min postadministration of radioactivity, and retention of label was evident through 24 hr. Coincident regions of abnormal accumulation were apparent with In-111-lgG, but only in delayed images, Moreover, with In-111-biotin alone, without the preadministration of streptavidin, focal accumulations were detected in areas similar to that identified with the two-step protocol. Although, these observations were only in the earliest images. Conclusion: The results of this preliminary clinical investigation suggest that a two-step protocol with unlabeled streptavidin and radiolabeled biotin may be an alternative for the detection of infection
Articolo in rivista - Articolo scientifico
Humans; Aged; Streptavidin; Indium Radioisotopes; Bacterial Proteins; Aged, 80 and over; Adult; Chronic Disease; Middle Aged; Biotin; Female; Male; Osteomyelitis
English
1996
37
10
1655
1662
none
Rusckowski, M., Paganelli, G., Hnatowich, D., Magnani, P., Virzi, F., Fogarasi, M., et al. (1996). Imaging osteomyelitis with streptavidin and Indium-111-labeled biotin. THE JOURNAL OF NUCLEAR MEDICINE, 37(10), 1655-1662.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/35186
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