The published information on glutamate levels in cerebrospinal fluid (CSF) and modifications in neurological disorders is controversial. In the present study, we demonstrated a metabolic instability of glutamate in untreated CSF and a spurious elevation of its levels by the current methods of CSF acidification. These findings may explain the discrepancies observed with different methods of CSF processing and analysis. We suggest a method of inactivating CSF enzymes that yields stable glutamate levels under different storage conditions. Use of such a method may be necessary for clinical studies.

Ferrarese, C., Pecora, N., Frigo, M., Appollonio, I., & Frattola, L. (1993). Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid. ANNALS OF NEUROLOGY, 33(3), 316-319 [10.1002/ana.410330316].

Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid

FERRARESE, CARLO;PECORA, NICOLETTA;APPOLLONIO, ILDEBRANDO;
1993-03

Abstract

The published information on glutamate levels in cerebrospinal fluid (CSF) and modifications in neurological disorders is controversial. In the present study, we demonstrated a metabolic instability of glutamate in untreated CSF and a spurious elevation of its levels by the current methods of CSF acidification. These findings may explain the discrepancies observed with different methods of CSF processing and analysis. We suggest a method of inactivating CSF enzymes that yields stable glutamate levels under different storage conditions. Use of such a method may be necessary for clinical studies.
Articolo in rivista - Articolo scientifico
Scientifica
Cerebrospinal Fluid; Peripheral Nervous System Diseases; Methods; Humans; Glutamic Acid; Acids; Glutamates
English
Ferrarese, C., Pecora, N., Frigo, M., Appollonio, I., & Frattola, L. (1993). Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid. ANNALS OF NEUROLOGY, 33(3), 316-319 [10.1002/ana.410330316].
Ferrarese, C; Pecora, N; Frigo, M; Appollonio, I; Frattola, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/35060
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