The published information on glutamate levels in cerebrospinal fluid (CSF) and modifications in neurological disorders is controversial. In the present study, we demonstrated a metabolic instability of glutamate in untreated CSF and a spurious elevation of its levels by the current methods of CSF acidification. These findings may explain the discrepancies observed with different methods of CSF processing and analysis. We suggest a method of inactivating CSF enzymes that yields stable glutamate levels under different storage conditions. Use of such a method may be necessary for clinical studies.

Ferrarese, C., Pecora, N., Frigo, M., Appollonio, I., Frattola, L. (1993). Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid. ANNALS OF NEUROLOGY, 33(3), 316-319 [10.1002/ana.410330316].

Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid

FERRARESE, CARLO;PECORA, NICOLETTA;APPOLLONIO, ILDEBRANDO;
1993

Abstract

The published information on glutamate levels in cerebrospinal fluid (CSF) and modifications in neurological disorders is controversial. In the present study, we demonstrated a metabolic instability of glutamate in untreated CSF and a spurious elevation of its levels by the current methods of CSF acidification. These findings may explain the discrepancies observed with different methods of CSF processing and analysis. We suggest a method of inactivating CSF enzymes that yields stable glutamate levels under different storage conditions. Use of such a method may be necessary for clinical studies.
Articolo in rivista - Articolo scientifico
Cerebrospinal Fluid; Peripheral Nervous System Diseases; Methods; Humans; Glutamic Acid; Acids; Glutamates
English
316
319
4
Ferrarese, C., Pecora, N., Frigo, M., Appollonio, I., Frattola, L. (1993). Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid. ANNALS OF NEUROLOGY, 33(3), 316-319 [10.1002/ana.410330316].
Ferrarese, C; Pecora, N; Frigo, M; Appollonio, I; Frattola, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/35060
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