In Saccharomyces cerevisiae maltose utilization requires a functional MAL locus, each composed of three genes: MALR (gene 3) encoding a regulatory protein, MALT (gene 1) encoding maltose permease and MALS (gene 2) encoding maltase. We show that constitutive activation of the RAS/protein kinase A pathway severely reduces growth of MAL1 strains on maltose. This may be a consequence of reduction in MALT mRNA, reduced Vmax and increased catabolite inactivation of the MALT-encoded maltose transporter in the MAL1 strain. Mutations in the GGS1/TPS1 gene, which restricts glucose influx and possibly affects signalling, relieve carbon catabolite repression on both maltase and maltose permease and reduce maltose permease inactivation

Wanke, V., Vavassori, M., Thevelein, J., Tortora, P., & Vanoni, M. (1997). Regulation of maltose utilization in Saccharomyces cerevisiae by genes of the RAS/protein kinase A pathway. FEBS LETTERS, 402(2-3), 251-255 [10.1016/S0014-5793(97)00009-4].

Regulation of maltose utilization in Saccharomyces cerevisiae by genes of the RAS/protein kinase A pathway

WANKE, VALERIA;TORTORA, PAOLO;VANONI, MARCO ERCOLE
1997

Abstract

In Saccharomyces cerevisiae maltose utilization requires a functional MAL locus, each composed of three genes: MALR (gene 3) encoding a regulatory protein, MALT (gene 1) encoding maltose permease and MALS (gene 2) encoding maltase. We show that constitutive activation of the RAS/protein kinase A pathway severely reduces growth of MAL1 strains on maltose. This may be a consequence of reduction in MALT mRNA, reduced Vmax and increased catabolite inactivation of the MALT-encoded maltose transporter in the MAL1 strain. Mutations in the GGS1/TPS1 gene, which restricts glucose influx and possibly affects signalling, relieve carbon catabolite repression on both maltase and maltose permease and reduce maltose permease inactivation
Articolo in rivista - Articolo scientifico
ras Proteins; Genes, Fungal; Membrane Transport Proteins; Recombinant Proteins; Transcription, Genetic; Saccharomyces cerevisiae; RNA, Messenger; Kinetics; alpha-Glucosidases; Cyclic AMP-Dependent Protein Kinases; Trans-Activators; Monosaccharide Transport Proteins; Maltose
English
251
255
5
Wanke, V., Vavassori, M., Thevelein, J., Tortora, P., & Vanoni, M. (1997). Regulation of maltose utilization in Saccharomyces cerevisiae by genes of the RAS/protein kinase A pathway. FEBS LETTERS, 402(2-3), 251-255 [10.1016/S0014-5793(97)00009-4].
Wanke, V; Vavassori, M; Thevelein, J; Tortora, P; Vanoni, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/34339
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