The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric beta-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1-10 strong promoter elements. It has a molecular weight of 180,000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255-1550 the protein is found in the soluble fraction

Vanoni, M., Vavassori, M., Frascotti, G., Martegani, E., & Alberghina, L. (1990). Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 172(1), 61-69 [10.1016/S0006-291X(05)80173-1].

Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product

VANONI, MARCO ERCOLE;FRASCOTTI, GIANNI;MARTEGANI, ENZO;ALBERGHINA, LILIA
1990

Abstract

The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric beta-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1-10 strong promoter elements. It has a molecular weight of 180,000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255-1550 the protein is found in the soluble fraction
No
Articolo in rivista - Articolo scientifico
Scientifica
Gene Expression Regulation, Fungal; Immunoblotting; ras-GRF1; Cell Cycle Proteins; ras Proteins; beta-Galactosidase; Genes, Fungal; Electrophoresis, Polyacrylamide Gel; Saccharomyces cerevisiae Proteins; Adenylate Cyclase; Recombinant Fusion Proteins; Fungal Proteins; Molecular Weight; Saccharomyces cerevisiae; Cloning, Molecular; Antibodies; Restriction Mapping
English
61
69
9
Vanoni, M., Vavassori, M., Frascotti, G., Martegani, E., & Alberghina, L. (1990). Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 172(1), 61-69 [10.1016/S0006-291X(05)80173-1].
Vanoni, M; Vavassori, M; Frascotti, G; Martegani, E; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/34328
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