The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric beta-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1-10 strong promoter elements. It has a molecular weight of 180,000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255-1550 the protein is found in the soluble fraction
Vanoni, M., Vavassori, M., Frascotti, G., Martegani, E., & Alberghina, L. (1990). Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 172(1), 61-69 [10.1016/S0006-291X(05)80173-1].
Citazione: | Vanoni, M., Vavassori, M., Frascotti, G., Martegani, E., & Alberghina, L. (1990). Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 172(1), 61-69 [10.1016/S0006-291X(05)80173-1]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product | |
Autori: | Vanoni, M; Vavassori, M; Frascotti, G; Martegani, E; Alberghina, L | |
Autori: | ||
Data di pubblicazione: | 1990 | |
Lingua: | English | |
Rivista: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0006-291X(05)80173-1 | |
Appare nelle tipologie: | 01 - Articolo su rivista |