Any one of three homologous genes - STA1, STA2 and STA3 - encoding glucoamylase isozymes I, II and III respectively, allows the Saccharomyces species to utilize starch as a sole carbon source. We show in this paper that glucoamylase II production can be increased 4-fold over the level produced by STA2 strains, by using a two-step fermentation and a yeast strain transformed with a high-copy-number plasmid carrying the STA2 gene. The accumulation of anomalous STA2 mRNA species, mainly differing at their 5' ends, and saturation of step(s) in the secretory pathway appear to be among the major factors limiting glucoamylase expression in synthetic media

Vanoni, M., Lotti, M., & Alberghina, L. (1989). Expression of cloned Saccharomyces diastaticus glucoamylase under natural and inducible promoters. BIOCHIMICA ET BIOPHYSICA ACTA, 1008(2), 168-176 [10.1016/0167-4781(80)90004-4].

Expression of cloned Saccharomyces diastaticus glucoamylase under natural and inducible promoters

VANONI, MARCO ERCOLE;LOTTI, MARINA;ALBERGHINA, LILIA
1989

Abstract

Any one of three homologous genes - STA1, STA2 and STA3 - encoding glucoamylase isozymes I, II and III respectively, allows the Saccharomyces species to utilize starch as a sole carbon source. We show in this paper that glucoamylase II production can be increased 4-fold over the level produced by STA2 strains, by using a two-step fermentation and a yeast strain transformed with a high-copy-number plasmid carrying the STA2 gene. The accumulation of anomalous STA2 mRNA species, mainly differing at their 5' ends, and saturation of step(s) in the secretory pathway appear to be among the major factors limiting glucoamylase expression in synthetic media
Articolo in rivista - Articolo scientifico
Scientifica
Immunoblotting; Glucan 1,4-alpha-Glucosidase; Genes, Fungal; Electrophoresis, Polyacrylamide Gel; Fermentation; Multigene Family; RNA, Fungal; Plasmids; Cloning, Molecular; Nucleotide Mapping; RNA, Messenger; Promoter Regions, Genetic; Saccharomyces; Transformation, Genetic; Culture Media; Escherichia coli; Isoenzymes; Gene Expression Regulation
English
Vanoni, M., Lotti, M., & Alberghina, L. (1989). Expression of cloned Saccharomyces diastaticus glucoamylase under natural and inducible promoters. BIOCHIMICA ET BIOPHYSICA ACTA, 1008(2), 168-176 [10.1016/0167-4781(80)90004-4].
Vanoni, M; Lotti, M; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/34321
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